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A reference linkage map for Eucalyptus

Citation

Hudson, CJ and Freeman, JS and Kullan, ARK and Petroli, CD and Sansaloni, CP and Kilian, A and Detering, F and Grattapaglia, D and Potts, BM and Myburg, AA and Vaillancourt, RE, A reference linkage map for Eucalyptus, BMC Genomics, 13, (1) Article 240. ISSN 1471-2164 (2012) [Refereed Article]


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Licenced under Creative Commons Attribution 2.0 Generic (CC BY 2.0) http://creativecommons.org/licenses/by/2.0/

DOI: doi:10.1186/1471-2164-13-240

Abstract

Background: Genetic linkage maps are invaluable resources in plant research. They provide a key tool for many genetic applications including: mapping quantitative trait loci (QTL); comparative mapping; identifying unlinked (i.e. independent) DNA markers for fingerprinting, population genetics and phylogenetics; assisting genome sequence assembly; relating physical and recombination distances along the genome and map-based cloning of genes. Eucalypts are the dominant tree species in most Australian ecosystems and of economic importance globally as plantation trees. The genome sequence of E. grandis has recently been released providing unprecedented opportunities for genetic and genomic research in the genus. A robust reference linkage map containing sequence-based molecular markers is needed to capitalise on this resource. Several high density linkage maps have recently been constructed for the main commercial forestry species in the genus (E. grandis, E. urophylla and E. globulus) using sequenced Diversity Arrays Technology (DArT) and microsatellite markers. To provide a single reference linkage map for eucalypts a composite map was produced through the integration of data from seven independent mapping experiments (1950 individuals) using a marker-merging method. Results: The composite map totalled 1107 cM and contained 4101 markers; comprising 3880 DArT, 213 microsatellite and eight candidate genes. Eighty-one DArT markers were mapped to two or more linkage groups, resulting in the 4101 markers being mapped to 4191 map positions. Approximately 13% of DArT markers mapped to identical map positions, thus the composite map contained 3634 unique loci at an average interval of 0.31 cM. Conclusion: The composite map represents the most saturated linkage map yet produced in Eucalyptus. As the majority of DArT markers contained on the map have been sequenced, the map provides a direct link to the E. grandis genome sequence and will serve as an important reference for progressing eucalypt research.

Item Details

Item Type:Refereed Article
Keywords:genomic, eucalypt, linkage map
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Population, Ecological and Evolutionary Genetics
Objective Division:Plant Production and Plant Primary Products
Objective Group:Forestry
Objective Field:Hardwood Plantations
Author:Hudson, CJ (Mr Corey Hudson)
Author:Freeman, JS (Dr Jules Freeman)
Author:Potts, BM (Professor Brad Potts)
Author:Vaillancourt, RE (Professor Rene Vaillancourt)
ID Code:80168
Year Published:2012
Funding Support:Australian Research Council (DP110101621)
Web of Science® Times Cited:23
Deposited By:Plant Science
Deposited On:2012-10-24
Last Modified:2017-11-03
Downloads:219 View Download Statistics

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