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Loss of TNF signaling facilitates the development of a novel Ly-6Clow macrophage population permissive for Leishmania major infection

Citation

Fromm, PD and Kling, J and Mack, M and Sedgwick, JD and Korner, H, Loss of TNF signaling facilitates the development of a novel Ly-6Clow macrophage population permissive for Leishmania major infection, Journal of Immunology, 188, (12) pp. 6258-6266. ISSN 0022-1767 (2012) [Refereed Article]


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Copyright Statement

Copyright 2012 by The American Association of Immunologists, Inc

Official URL: http://www.jimmunol.org/content/188/12.toc

DOI: doi:10.4049/jimmunol.1100977

Abstract

In the absence of TNF, the normally resistant C57BL/6 (B6.WT) strain develops a fatal, progressive form of leishmaniasis after infection with Leishmania major. It is not yet understood which TNF activity or the lack thereof is responsible for the dramatic progression of leishmaniasis in TNF-negative (B6.TNF-/-) mice. To elucidate the underlying mechanisms resulting in the fatal outcome of L. major infection in this gene-deficient mouse strain, we analyzed the monocytic component of the inflammatory infiltrate in the draining popliteal lymph node and the site of the infection using multicolor flow cytometry. The leukocytic infiltrate within the draining lymph node and footpad of B6.TNF-/- mice resembled that of B6.WT mice over the first 2 wk of cutaneous L. major infection. Thereafter, the B6.TNF-/- mice showed an increase of CD11c+Ly-6C+CCR2+ monocytic dendritic cells within the popliteal lymph node in comparison with B6.WT mice. This increase of inflammatory dendritic cells was paired with the accumulation of a novel CD11b+Ly-6ClowCCR2low population that was not present in B6.WT mice. This B6.TNF-/-- and B6.TNFR1-/--specific cell population was CD115+Ly-6G-iNOS-, not apoptotic, and harbored large numbers of parasites.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Immunology not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Kling, J (Miss Jessica Kling)
Author:Korner, H (Professor Heinrich Korner)
ID Code:80049
Year Published:2012
Web of Science® Times Cited:9
Deposited By:Menzies Institute for Medical Research
Deposited On:2012-10-18
Last Modified:2017-11-07
Downloads:0

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