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Quantifying kinetics of net ion fluxes from plant tissues by non-invasive microelectrode measuring MIFE technique


Shabala, S and Cuin, TA and Shabala, L and Newman, I, Quantifying kinetics of net ion fluxes from plant tissues by non-invasive microelectrode measuring MIFE technique, Plant Salt Tolerance: Methods and Protocols, Humana Press, S Shabala and TA Cuin (ed), New York, pp. 119-134. ISBN 978-1-61779-985-3 (2012) [Research Book Chapter]

Copyright Statement

Copyright 2012 Springer Science+Business Media, LLC

DOI: doi:10.1007/978-1-61779-986-0


Non-invasive microelectrode ion fl ux measuring (the MIFE system) allows concurrent quantification of net fluxes of several ions with high spatial (several μm) and temporal (ca 5 s) resolution. Over the last 10 years, the MIFE system has been widely used to study various aspects of salt stress signaling and adaptation in plants. This chapter summarizes some major findings in the area such as using MIFE for deciphering the specific and non-specific components of salinity stress, resolving the role of the plasma membrane H+-pump in salinity responses, proving K+ homeostasis as a key feature of salinity tolerance, and discovering the mechanisms behind the ameliorative effects of Ca2+ and other mitigating factors (such as polyamines or compatible solutes). The full protocols for microelectrode fabrication, calibration, and use are then given, and two basic routines for measuring net K+ and Na+ fluxes from salinity stressed roots are described in the context of plant screening for salt stress tolerance.

Item Details

Item Type:Research Book Chapter
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other plant production and plant primary products
Objective Field:Other plant production and plant primary products not elsewhere classified
UTAS Author:Shabala, S (Professor Sergey Shabala)
UTAS Author:Cuin, TA (Dr Tracey Cuin)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Newman, I (Dr Ian Newman)
ID Code:80038
Year Published:2012
Deposited By:Agricultural Science
Deposited On:2012-10-17
Last Modified:2018-07-05
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