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Interdomain orientation of cardiac Troponin C characterized by paramagnetic relaxation enhancement NMR reveals a compact state
Citation
Cordina, NM and Liew, CK and Gell, DA and Fajer, PG and Mackay, JP and Brown, LJ, Interdomain orientation of cardiac Troponin C characterized by paramagnetic relaxation enhancement NMR reveals a compact state, Protein Science, 21, (9) pp. 1376-1387. ISSN 1469-896X (2012) [Refereed Article]
Copyright Statement
Copyright 2012 The Protein Society
DOI: doi:10.1002/pro.2124
Abstract
Cardiac troponin C (cTnC) is the calcium binding subunit of the troponin complex that triggers the thin filament response to calcium influx into the sarcomere. cTnC consists of two globular EF-hand domains (termed the N- and C-domains) connected by a flexible linker. While the conformation of each domain of cTnC has been thoroughly characterized through NMR studies involving either the isolated N-domain (N-cTnC) or C-domain (C-cTnC), little attention has been paid to the range of interdomain orientations possible in full-length cTnC that arises as a consequence of the flexibility of the domain linker. Flexibility in the domain linker of cTnC is essential for effective regulatory function of troponin. We have therefore utilized paramagnetic relaxation enhancement (PRE) NMR to assess the interdomain orientation of cTnC. Ensemble fitting of our interdomain PRE measurements reveals that isolated cTnC has considerable interdomain flexibility and preferentially adopts a bent conformation in solution, with a defined range of relative domain orientations.
Item Details
Item Type: | Refereed Article |
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Keywords: | cardiac troponin C, solution NMR, paramagnetic relaxation enhancement, site-directed spin labeling, ensemble states |
Research Division: | Biological Sciences |
Research Group: | Biochemistry and cell biology |
Research Field: | Structural biology (incl. macromolecular modelling) |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Gell, DA (Dr David Gell) |
ID Code: | 79772 |
Year Published: | 2012 |
Web of Science® Times Cited: | 13 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2012-10-02 |
Last Modified: | 2018-03-30 |
Downloads: | 0 |
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