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Properties and Fate of Oligodendrocyte Progenitor Cells in the Corpus Callosum, Motor Cortex, and Piriform Cortex of the Mouse

Citation

Clarke, LE and Young, KM and Hamilton, NB and Li, H and Richardson, WD and Attwell, D, Properties and Fate of Oligodendrocyte Progenitor Cells in the Corpus Callosum, Motor Cortex, and Piriform Cortex of the Mouse, Journal of Neuroscience, 32, (24) pp. 8173-8185. ISSN 0270-6474 (2012) [Refereed Article]


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Copyright 2012 The Authors

DOI: doi:10.1523/JNEUROSCI.0928-12.2012

Abstract

Oligodendrocyte progenitor cells (OPCs) in the postnatal mouse corpus callosum (CC) and motor cortex (Ctx) reportedly generate only oligodendrocytes (OLs), whereas those in the piriform cortex may also generate neurons. OPCs have also been subdivided based on their expression of voltage-gated ion channels, ability to respond to neuronal activity, and proliferative state. To determine whether OPCs in the piriform cortex have inherently different physiological properties from those in the CC and Ctx, we studied acute brain slices from postnatal transgenic mice in which GFP expression identifies OL lineage cells. We whole-cell patch clamped GFP-expressing (GFP+) cells within the CC, Ctx, and anterior piriform cortex (aPC) and used prelabeling with 5-ethynyl-2'-deoxyuridine (EdU) to assess cell proliferation. After recording, slices were immunolabeled and OPCs were defined by strong expression of NG2. NG2+ OPCs in the white and gray matter proliferated and coexpressed PDGFRα and voltage-gated Na+ channels (INa). Approximately 70% of OPCs were capable of generating regenerative depolarizations. In addition to OLIG2+ NG2+ INa+ OPCs and OLIG2+ NG2neg INaneg OLs, we identified cells with low levels of NG2 limited to the soma or the base of some processes. These cells had a significantly reduced INa and a reduced ability to incorporate EdU when compared with OPCs and probably correspond to early differentiating OLs. By combining EdU labeling and lineage tracing using Pdgfrα-CreERT2:R26R-YFP transgenic mice, we double labeled OPCs and traced their fate in the postnatal brain. These OPCs generated OLs but did not generate neurons in the aPC or elsewhere at any time that we examined.

Item Details

Item Type:Refereed Article
Keywords:oligodendrocyte, proliferation, voltage gated sodium channels
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Young, KM (Dr Kaylene Young)
ID Code:79462
Year Published:2012
Funding Support:National Health and Medical Research Council (1030939)
Web of Science® Times Cited:74
Deposited By:Menzies Institute for Medical Research
Deposited On:2012-09-12
Last Modified:2013-04-17
Downloads:0

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