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Characterization of the amino acids essential for the photo- and radioprotective effects of a Bowman-Birk protease inhibitor -derived nonapeptide

Citation

Dittmann, KH and Gueven, N and Mayer, C and Rodemann, HP, Characterization of the amino acids essential for the photo- and radioprotective effects of a Bowman-Birk protease inhibitor -derived nonapeptide, Protein Engineering, 14, (3) pp. 157-160. ISSN 0269-2139 (2001) [Refereed Article]

Copyright Statement

Copyright 2001 Oxford University Press

DOI: doi:10.1093/protein/14.3.157

Abstract

The Bowman-Birk protease inhibitor has been reported to exert photo- and radioprotective activity. This effect was assigned to a cyclic nonapeptide sequence which is known to contain the amino acids responsible for the anti-chymotryptic activity of the BBI. The present study indicated that linearization of the nonapeptide resulted in a significant loss of anti-proteolytic activity, whereas the photo- and radioprotective capacity persisted. Substitution of the amino acids Leu or Ser of the nonapeptide, essential for the anti-proteolytic activity, with different amino acids, indicated that rather the hydrophobic features of the amino acids in this position than charge are critical to retain the photo- and radioprotective effect. These results suggest the existence of a bifunctional peptide sequence with anti-proteolytic and photo-/radioprotective capacity. However, the lack of correlation between the photo-/radioprotective activity and the anti-proteolytic activity within the peptides generated by modification of the linear nonapeptide argues for the existence of two closely colocalized domains within the nonapeptide responsible for photo-/radioprotection and protease inhibition.

Item Details

Item Type:Refereed Article
Keywords:BBI, Bowman Birk Protease inhibitor, radioprotection
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Signal Transduction
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Gueven, N (Dr Nuri Guven)
ID Code:79325
Year Published:2001
Web of Science® Times Cited:14
Deposited By:Pharmacy
Deposited On:2012-08-31
Last Modified:2014-11-05
Downloads:0

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