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Functional link between BLM defective in Bloom's syndrome and the ataxia-telangiectasia-mutated protein, ATM

Citation

Beamish, H and Kedar, P and Kaneko, H and Chen, P and Fukao, T and Peng, C and Beresten, S and Gueven, N and Purdie, D and Lees-Miller, S and Ellis, N and Kondo, N and Lavin, MF, Functional link between BLM defective in Bloom's syndrome and the ataxia-telangiectasia-mutated protein, ATM, Journal of Biological Chemistry, 277, (34) pp. 30515-30523. ISSN 0021-9258 (2002) [Refereed Article]


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Licensed under Creative Commons Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/

DOI: doi:10.1074/jbc.M203801200

Abstract

Chromosome aberrations, genomic instability, and cancer predisposition are hallmarks of a number of syndromes in which the defective genes recognize and/or repair DNA damage or are involved in some aspect of DNA processing. We report here direct interaction between BLM, mutated in Bloom's Syndrome (BS), and ATM, mutated is ataxia-telangiectasia, and we have mapped the sites of interaction. Full-length BLM cDNA corrected sister chromatid exchange (SCE) and radiosensitivity in BS cells. Mitotic phosphorylation of BLM was partially dependent on ATM, and phosphorylation sites on BLM were identified. A phosphospecific antibody against one of these sites (Thr-99) revealed radiation-induced phosphorylation, which was defective in ataxia-telangiectasia cells. Stable cell lines expressing phosphorylation site mutants failed to correct radiosensitivity in BS cells but corrected SCE. These mutants also sensitized normal control cells to radiation and increased radiation-induced chromosome aberrations but did not cause SCE numbers to increase. These data suggest that ATM and BLM function together in recognizing abnormal DNA structures by direct interaction and that these phosphorylation sites in BLM are important for radiosensitivity status but not for SCE frequency.

Item Details

Item Type:Refereed Article
Keywords:radiosensitivty, ataxia-telangiectasia
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Signal Transduction
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Gueven, N (Dr Nuri Guven)
ID Code:79324
Year Published:2002
Web of Science® Times Cited:92
Deposited By:Pharmacy
Deposited On:2012-08-31
Last Modified:2016-10-19
Downloads:195 View Download Statistics

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