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A novel form of ataxia oculomotor apraxia characterized by oxidative stress and apoptosis resistance
journal contribution
posted on 2023-05-17, 13:02 authored by Nuri GuvenNuri Guven, Becherel, OJ, Howe, O, Chen, P, Haince, J-F, Ouellet, M-E, Poirier, GG, Waterhouse, N, Fusser, M, Epe, B, de Murcia, JM, de Murcia, G, McGowan, CH, Parton, R, Mothersill, C, Grattan-Smith, P, Lavin, MFSeveral different autosomal recessive genetic disorders characterized by ataxia with oculomotor apraxia (AOA) have been identified with the unifying feature of defective DNA damage recognition and/or repair. We describe here the characterization of a novel form of AOA showing increased sensitivity to agents that cause single-strand breaks (SSBs) in DNA but having no gross defect in the repair of these breaks. Evidence for the presence of residual SSBs in DNA was provided by dramatically increased levels of poly (ADP-ribose)polymerase (PARP-1) auto-poly (ADP-ribosyl)ation, the detection of increased levels of reactive oxygen/nitrogen species (ROS/RNS) and oxidative damage to DNA in the patient cells. There was also evidence for oxidative damage to proteins and lipids. Although these cells were hypersensitive to DNA damaging agents, the mode of death was not by apoptosis. These cells were also resistant to TRAIL-induced death. Consistent with these observations, failure to observe a decrease in mitochondrial membrane potential, reduced cytochrome c release and defective apoptosis-inducing factor translocation to the nucleus was observed. Apoptosis resistance and PARP-1 hyperactivation were overcome by incubating the patient's cells with antioxidants. These results provide evidence for a novel form of AOA characterized by sensitivity to DNA damaging agents, oxidative stress, PARP-1 hyperactivation but resistance to apoptosis.
History
Publication title
Cell Death and DifferentiationVolume
14Issue
6Pagination
1149-1161ISSN
1350-9047Department/School
School of Pharmacy and PharmacologyPublisher
Nature Publishing GroupPlace of publication
Macmillan Building, 4 Crinan St, London, England, N1 9XwRights statement
Copyright 2007 Nature Publishing GroupRepository Status
- Restricted