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A novel profluorescent nitroxide as a sensitive probe for the cellular redox environment

Citation

Morrow, BJ and Keddie, DJ and Gueven, N and Lavin, MF and Bottle, SE, A novel profluorescent nitroxide as a sensitive probe for the cellular redox environment, Free Radical Biology & Medicine, 49, (1) pp. 67-76. ISSN 1873-4596 (2010) [Refereed Article]

Copyright Statement

Copyright 2012 Elsevier

DOI: doi:10.1016/j.freeradbiomed.2010.03.019

Abstract

Changes to the redox status of biological systems have been implicated in the pathogenesis of a wide variety of disorders. Sensitive quantification of these changes has been developed using a novel fluorescent probe containing a redox-sensitive nitroxide moiety. As well as being able to selectively detect the superoxide radical in vitro, this method can measure overall changes to the cellular redox environment using flow cytometry on the basis of nitroxide reduction. The reversible nature of the probe's detection mechanism offers the unique advantage of being able to monitor redox changes in both oxidizing and reducing directions in real time.

Item Details

Item Type:Refereed Article
Keywords:nitroxide, oxidative stress, fluorescent probe, free radicals
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Analytical Chemistry not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Gueven, N (Dr Nuri Guven)
ID Code:79274
Year Published:2010
Web of Science® Times Cited:36
Deposited By:Pharmacy
Deposited On:2012-08-29
Last Modified:2013-03-22
Downloads:0

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