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MudPIT profiling reveals a link between anaerobic metabolism and the alkaline adaptive response of Listeria monocytogenes EGD-e

Citation

Nilsson, RE and Ross, T and Bowman, JP and Britz, ML, MudPIT profiling reveals a link between anaerobic metabolism and the alkaline adaptive response of Listeria monocytogenes EGD-e, PLoS One, 8, (1) Article e54157. ISSN 1932-6203 (2013) [Refereed Article]


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Copyright Statement

Copyright 2013 Nilsson et al. Licensed under Creative Commons Attribution License 2.5 Generic (CC BY 2.5) http://creativecommons.org/licenses/by/2.5/

DOI: doi:10.1371/journal.pone.0054157

Abstract

Listeria monocytogenes is a foodborne human pathogen capable of causing life-threatening disease in susceptible populations. Previous proteomic analysis we performed demonstrated that different strains of L. monocytogenes initiate a stringent response when subjected to alkaline growth conditions. Here, using multidimensional protein identification technology (MudPIT), we show that in L. monocytogenes EGD-e this response involves an energy shift to anaerobic pathways in response to the extracellular pH environment. Importantly we show that this supports a reduction in relative lag time following an abrupt transition to low oxygen tension culture conditions. This has important implications for the packaging of fresh and ready-to-eat foods under reduced oxygen conditions in environments where potential exists for alkaline adaptation.

Item Details

Item Type:Refereed Article
Keywords:MudPIT, anaerobic metabolism, Listeria monocytogenes
Research Division:Engineering
Research Group:Food Sciences
Research Field:Food Packaging, Preservation and Safety
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Food Safety
Author:Nilsson, RE (Mr Rolf Nilsson)
Author:Ross, T (Associate Professor Tom Ross)
Author:Bowman, JP (Associate Professor John Bowman)
Author:Britz, ML (Professor Margaret Britz)
ID Code:78906
Year Published:2013
Web of Science® Times Cited:7
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2012-08-04
Last Modified:2014-04-03
Downloads:770 View Download Statistics

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