R Nilsson etal journal.pone.0054157.pdf (899.14 kB)
MudPIT profiling reveals a link between anaerobic metabolism and the alkaline adaptive response of Listeria monocytogenes EGD-e
journal contribution
posted on 2023-05-17, 12:46 authored by Nilsson, RE, Thomas RossThomas Ross, John BowmanJohn Bowman, Margaret BritzMargaret BritzListeria monocytogenes is a foodborne human pathogen capable of causing life-threatening disease in susceptible populations. Previous proteomic analysis we performed demonstrated that different strains of L. monocytogenes initiate a stringent response when subjected to alkaline growth conditions. Here, using multidimensional protein identification technology (MudPIT), we show that in L. monocytogenes EGD-e this response involves an energy shift to anaerobic pathways in response to the extracellular pH environment. Importantly we show that this supports a reduction in relative lag time following an abrupt transition to low oxygen tension culture conditions. This has important implications for the packaging of fresh and ready-to-eat foods under reduced oxygen conditions in environments where potential exists for alkaline adaptation.
History
Publication title
PLoS OneVolume
8Article number
e54157Number
e54157Pagination
1-9ISSN
1932-6203Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Public Library of SciencePlace of publication
San Franciso, USARights statement
Copyright 2013 Nilsson et al. Licensed under Creative Commons Attribution License 2.5 Generic (CC BY 2.5) http://creativecommons.org/licenses/by/2.5/Repository Status
- Open