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MudPIT profiling reveals a link between anaerobic metabolism and the alkaline adaptive response of Listeria monocytogenes EGD-e

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posted on 2023-05-17, 12:46 authored by Nilsson, RE, Thomas RossThomas Ross, John BowmanJohn Bowman, Margaret BritzMargaret Britz
Listeria monocytogenes is a foodborne human pathogen capable of causing life-threatening disease in susceptible populations. Previous proteomic analysis we performed demonstrated that different strains of L. monocytogenes initiate a stringent response when subjected to alkaline growth conditions. Here, using multidimensional protein identification technology (MudPIT), we show that in L. monocytogenes EGD-e this response involves an energy shift to anaerobic pathways in response to the extracellular pH environment. Importantly we show that this supports a reduction in relative lag time following an abrupt transition to low oxygen tension culture conditions. This has important implications for the packaging of fresh and ready-to-eat foods under reduced oxygen conditions in environments where potential exists for alkaline adaptation.

History

Publication title

PLoS One

Volume

8

Article number

e54157

Number

e54157

Pagination

1-9

ISSN

1932-6203

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Public Library of Science

Place of publication

San Franciso, USA

Rights statement

Copyright 2013 Nilsson et al. Licensed under Creative Commons Attribution License 2.5 Generic (CC BY 2.5) http://creativecommons.org/licenses/by/2.5/

Repository Status

  • Open

Socio-economic Objectives

Food safety

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