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MudPIT profiling reveals a link between anaerobic metabolism and the alkaline adaptive response of Listeria monocytogenes EGD-e
Citation
Nilsson, RE and Ross, T and Bowman, JP and Britz, ML, MudPIT profiling reveals a link between anaerobic metabolism and the alkaline adaptive response of Listeria monocytogenes EGD-e, PLoS One, 8, (1) Article e54157. ISSN 1932-6203 (2013) [Refereed Article]
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Copyright Statement
Copyright 2013 Nilsson et al. Licensed under Creative Commons Attribution License 2.5 Generic (CC BY 2.5) http://creativecommons.org/licenses/by/2.5/
DOI: doi:10.1371/journal.pone.0054157
Abstract
Listeria monocytogenes is a foodborne human pathogen capable of causing life-threatening disease in susceptible populations. Previous proteomic analysis we performed demonstrated that different strains of L. monocytogenes initiate
a stringent response when subjected to alkaline growth conditions. Here, using multidimensional protein identification technology (MudPIT), we show that in L. monocytogenes EGD-e this response involves an energy shift to anaerobic pathways
in response to the extracellular pH environment. Importantly we show that this supports a reduction in relative lag time following an abrupt transition to low oxygen tension culture conditions. This has important implications for the packaging of fresh and ready-to-eat foods under reduced oxygen conditions in environments where potential exists for alkaline adaptation.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | MudPIT, anaerobic metabolism, Listeria monocytogenes |
| Research Division: | Agricultural, Veterinary and Food Sciences |
| Research Group: | Food sciences |
| Research Field: | Food packaging, preservation and processing |
| Objective Division: | Health |
| Objective Group: | Public health (excl. specific population health) |
| Objective Field: | Food safety |
| UTAS Author: | Nilsson, RE (Mr Rolf Nilsson) |
| UTAS Author: | Ross, T (Professor Tom Ross) |
| UTAS Author: | Bowman, JP (Associate Professor John Bowman) |
| UTAS Author: | Britz, ML (Professor Margaret Britz) |
| ID Code: | 78906 |
| Year Published: | 2013 |
| Web of Science® Times Cited: | 12 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2012-08-04 |
| Last Modified: | 2014-04-03 |
| Downloads: | 904 View Download Statistics |
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