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Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease
journal contribution
posted on 2023-05-17, 12:44 authored by Abbiss, H, Maker, GL, Gummer, J, Matthew SharmanMatthew Sharman, Phillips, JK, Boyce, M, Trengove, RDAIM: The purpose of this research was to use metabolomics to investigate the cystic phenotype in the Lewis polycystic kidney rat. METHODS: Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups. RESULTS: With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model. CONCLUSION: The findings of this study demonstrate the potential of metabolomics
History
Publication title
NephrologyVolume
17Pagination
104-110ISSN
1320-5358Department/School
School of Health SciencesPublisher
Blackwell Publishing AsiaPlace of publication
54 University St, P O Box 378, Carlton, Australia, Victoria, 3053Rights statement
Copyright 2011 Asian Pacific Society of NephrologyRepository Status
- Restricted