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Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease


Abbiss, H and Maker, GL and Gummer, J and Sharman, MJ and Phillips, JK and Boyce, M and Trengove, RD, Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease, Nephrology, 17, (2) pp. 104-110. ISSN 1320-5358 (2012) [Refereed Article]

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Copyright Statement

Copyright 2011 Asian Pacific Society of Nephrology

DOI: doi:10.1111/j.1440-1797.2011.01532.x


AIM: The purpose of this research was to use metabolomics to investigate the cystic phenotype in the Lewis polycystic kidney rat. METHODS: Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups. RESULTS: With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model. CONCLUSION: The findings of this study demonstrate the potential of metabolomics

Item Details

Item Type:Refereed Article
Keywords:biomarker, gas chromatography, metabolite, polycystic kidney disease, urine
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Nephrology and Urology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified
UTAS Author:Sharman, MJ (Dr Matt Sharman)
ID Code:78873
Year Published:2012
Web of Science® Times Cited:14
Deposited By:Health Sciences A
Deposited On:2012-07-31
Last Modified:2017-10-13

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