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AIM: The purpose of this research was to use metabolomics to investigate the cystic phenotype in the Lewis polycystic kidney rat. METHODS: Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups. RESULTS: With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model. CONCLUSION: The findings of this study demonstrate the potential of metabolomics

Item Type:	Refereed Article
Keywords:	biomarker, gas chromatography, metabolite, polycystic kidney disease, urine
Research Division:	Medical and Health Sciences
Research Group:	Clinical Sciences
Research Field:	Nephrology and Urology
Objective Division:	Health
Objective Group:	Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:	Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified
Author:	Sharman, MJ (Dr Matt Sharman)
ID Code:	78873
Year Published:	2012
Web of Science® Times Cited:	11
Deposited By:	Health Sciences A
Deposited On:	2012-07-31
Last Modified:	2017-10-13
Downloads:	0