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Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease
Citation
Abbiss, H and Maker, GL and Gummer, J and Sharman, MJ and Phillips, JK and Boyce, M and Trengove, RD, Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease, Nephrology, 17, (2) pp. 104-110. ISSN 1320-5358 (2012) [Refereed Article]
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Copyright Statement
Copyright 2011 Asian Pacific Society of Nephrology
DOI: doi:10.1111/j.1440-1797.2011.01532.x
Abstract
AIM: The purpose of this research was to use metabolomics to investigate the cystic phenotype in the Lewis polycystic kidney rat.
METHODS: Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups.
RESULTS: With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model.
CONCLUSION: The findings of this study demonstrate the potential of metabolomics
Item Details
Item Type: | Refereed Article |
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Keywords: | biomarker, gas chromatography, metabolite, polycystic kidney disease, urine |
Research Division: | Biomedical and Clinical Sciences |
Research Group: | Clinical sciences |
Research Field: | Nephrology and urology |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Sharman, MJ (Dr Matt Sharman) |
ID Code: | 78873 |
Year Published: | 2012 |
Web of Science® Times Cited: | 16 |
Deposited By: | Health Sciences A |
Deposited On: | 2012-07-31 |
Last Modified: | 2017-10-13 |
Downloads: | 0 |
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