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Microvascular blood flow responses to muscle contraction are not altered by high-fat feeding in rats

Citation

St-Pierre, P and Keith, LJ and Richards, SM and Rattigan, S and Keske, MA, Microvascular blood flow responses to muscle contraction are not altered by high-fat feeding in rats, Diabetes, Obesity and Metabolism, 14, (8) pp. 753-761. ISSN 1462-8902 (2012) [Refereed Article]


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Copyright Statement

Copyright 2012 Blackwell Publishing Ltd.

DOI: doi:10.1111/j.1463-1326.2012.01598.x

Abstract

Aim: Exercise and insulin each increase microvascular blood flow and enhance glucose disposal in skeletal muscle. We have reported that insulin-mediated microvascular recruitment in a diet-induced model of insulin resistance (high-fat feeding for 4 weeks) is markedly impaired; however, the effect of muscle contraction in this model has not been previously explored.

Methods: We fed rats either normal (ND, 10% calories from fat) or high-fat (HFD, 60% calories from fat) diets ad libitum for 48 weeks. Animals were then anaesthetized and one hindlimb electrically stimulated to contract at 0.05, 0.1 and 2 Hz (field stimulation, 30 V, 0.1 ms duration) in 15 min stepwise increments. Femoral artery blood flow (Transonic flow probe), muscle microvascular blood flow (hindleg metabolism of 1-methylxanthine and contrast-enhanced ultrasound) and muscle glucose disposal (uptake of radiolabelled 2-deoxy-d-glucose and hindleg glucose disappearance) were measured.

Results: Both ND and HFD rats received the same voltage across the leg and consequently developed the same muscle tension. Femoral artery blood flow in the contracting leg increased during 2 Hz contraction, but not during the lower frequencies and these effects were similar between ND and HFD rats. Muscle microvascular blood flow significantly increased in a contraction frequency-dependent manner, and preceded increases in total limb blood flow and these effects were similar between ND and HFD rats. Muscle glucose disposal was markedly elevated during 2 Hz contraction and was comparable between ND and HFD rats.

Conclusion: Contraction-mediated muscle microvascular recruitment and glucose uptake are not impaired in the HFD insulin resistant rat.

Item Details

Item Type:Refereed Article
Keywords:dietary fat, insulin resistance, microvascular perfusion, muscle glucose uptake
Research Division:Medical and Health Sciences
Research Group:Medical Physiology
Research Field:Systems Physiology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cardiovascular System and Diseases
Author:St-Pierre, P (Mr Philippe St Pierre)
Author:Keith, LJ (Miss Laura Keith)
Author:Richards, SM (Dr Stephen Richards)
Author:Rattigan, S (Professor Stephen Rattigan)
Author:Keske, MA (Dr Michelle Keske)
ID Code:78867
Year Published:2012
Funding Support:Australian Research Council (DP0877385)
Web of Science® Times Cited:7
Deposited By:Menzies Institute for Medical Research
Deposited On:2012-07-31
Last Modified:2015-08-07
Downloads:0

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