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Microvascular blood flow responses to muscle contraction are not altered by high-fat feeding in rats
Citation
St-Pierre, P and Keith, LJ and Richards, SM and Rattigan, S and Keske, MA, Microvascular blood flow responses to muscle contraction are not altered by high-fat feeding in rats, Diabetes, Obesity and Metabolism, 14, (8) pp. 753-761. ISSN 1462-8902 (2012) [Refereed Article]
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Copyright Statement
Copyright 2012 Blackwell Publishing Ltd.
DOI: doi:10.1111/j.1463-1326.2012.01598.x
Abstract
Aim: Exercise and insulin each increase microvascular blood flow and enhance glucose disposal in skeletal muscle. We have reported that insulin-mediated microvascular recruitment in a diet-induced model of insulin resistance (high-fat feeding for 4 weeks) is markedly impaired; however, the effect of muscle contraction in this model has not been previously explored.
Methods: We fed rats either normal (ND, 10% calories from fat) or high-fat (HFD, 60% calories from fat) diets ad libitum for 4–8 weeks. Animals were then anaesthetized and one hindlimb electrically stimulated to contract at 0.05, 0.1 and 2 Hz (field stimulation, 30 V, 0.1 ms duration) in 15 min stepwise increments. Femoral artery blood flow (Transonic flow probe), muscle microvascular blood flow (hindleg metabolism of 1-methylxanthine and contrast-enhanced ultrasound) and muscle glucose disposal (uptake of radiolabelled 2-deoxy-d-glucose and hindleg glucose disappearance) were measured.
Results: Both ND and HFD rats received the same voltage across the leg and consequently developed the same muscle tension. Femoral artery blood flow in the contracting leg increased during 2 Hz contraction, but not during the lower frequencies and these effects were similar between ND and HFD rats. Muscle microvascular blood flow significantly increased in a contraction frequency-dependent manner, and preceded increases in total limb blood flow and these effects were similar between ND and HFD rats. Muscle glucose disposal was markedly elevated during 2 Hz contraction and was comparable between ND and HFD rats.
Conclusion: Contraction-mediated muscle microvascular recruitment and glucose uptake are not impaired in the HFD insulin resistant rat.
Item Details
Item Type: | Refereed Article |
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Keywords: | dietary fat, insulin resistance, microvascular perfusion, muscle glucose uptake |
Research Division: | Biomedical and Clinical Sciences |
Research Group: | Medical physiology |
Research Field: | Systems physiology |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | St-Pierre, P (Mr Philippe St Pierre) |
UTAS Author: | Keith, LJ (Miss Laura Keith) |
UTAS Author: | Richards, SM (Dr Stephen Richards) |
UTAS Author: | Rattigan, S (Professor Stephen Rattigan) |
UTAS Author: | Keske, MA (Dr Michelle Keske) |
ID Code: | 78867 |
Year Published: | 2012 |
Funding Support: | Australian Research Council (DP0877385) |
Web of Science® Times Cited: | 16 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2012-07-31 |
Last Modified: | 2017-11-06 |
Downloads: | 0 |
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