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Studying Membrane Transport Processes by Non-invasive Microelectrodes: Basic Principles and Methods

Citation

Shabala, S and Shabala, L and Newman, I, Studying Membrane Transport Processes by Non-invasive Microelectrodes: Basic Principles and Methods, Plant Electrophysiology : Methods and Cell Electrophysiology, Springer, AG Volkov (ed), New York, USA, pp. 167-186. ISBN 978-3-642-29118-0 (2012) [Research Book Chapter]

Copyright Statement

COpyright 2012 Springer Berlin Heidelberg

DOI: doi:10.1007/978-3-642-29119-7

Abstract

Non-invasive microelectrode flux measurement is a convenient tool to study membrane transport processes in plants in situ. Its high resolution (a few microns in space and several seconds in time) enables real-time studies of membrane transport processes at various levels of plant structural organization, from intact organs to various tissues, single cells or protoplasts derived from these cells. Being non-invasive, the technique allows flux measurements over several hours or days, even from rapidly moving tissues such as growing or nutating plant axial organs. Importantly, fluxes of several ions and neutral molecules can be measured concurrently, enabling not only quantitative estimates of rates of measured processes, but also providing some valuable information about the stoichiometry between activities of various membrane transporters and their time dependence. In this review, we revise the principles of non-invasive ion and neutral molecule flux measurements using the MIFE™ technique and discuss some methodological aspects of manufacturing, calibrating, and using flux measuring microelectrodes.

Item Details

Item Type:Research Book Chapter
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally Sustainable Plant Production
Objective Field:Environmentally Sustainable Plant Production not elsewhere classified
Author:Shabala, S (Professor Sergey Shabala)
Author:Shabala, L (Dr Lana Shabala)
Author:Newman, I (Dr Ian Newman)
ID Code:78788
Year Published:2012
Deposited By:Agricultural Science
Deposited On:2012-07-26
Last Modified:2017-10-16
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