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Identification of a biological activity that supports maintenance and proliferation of pluripotent cells from the primitive ectoderm of the mouse

Citation

Rathjen, J and Washington, JM and Bettess, MD and Rathjen, PD, Identification of a biological activity that supports maintenance and proliferation of pluripotent cells from the primitive ectoderm of the mouse, Biology of Reproduction, 69, (6) pp. 1863-1871. ISSN 0006-3363 (2003) [Refereed Article]

DOI: doi:10.1095/biolreprod.103.017384

Abstract

Pluripotent cell development in the mammalian embryo results in the sequential formation of several developmentally distinct populations, inner cell mass, primitive ectoderm, and the primordial germ lineage. Factors within medium conditioned by HepG2 cells (MEDII) have been implicated in the formation and maintenance of primitive ectoderm from inner cell mass cells both in vitro and in vivo. Here we demonstrate that MEDII, but not LIF, is able to support the maintenance and proliferation in culture of pluripotent cells derived from primitive ectoderm formed in vitro or during embryonic development. This distinguishes primitive ectoderm and inner cell mass (ICM) on the basis of cytokine responsiveness and validates the biological activity proposed for factors within MEDII in primitive ectoderm establishment and maintenance. Further, it potentially provides an alternative technology for the isolation of pluripotent cells from the mammalian embryo.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Cell Development, Proliferation and Death
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Rathjen, J (Dr Joy Rathjen)
Author:Rathjen, PD (Professor Peter Rathjen)
ID Code:78734
Year Published:2003
Web of Science® Times Cited:10
Deposited By:Menzies Institute for Medical Research
Deposited On:2012-07-24
Last Modified:2012-07-24
Downloads:0

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