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Response to BMP4 signalling during ES cell differentiation defines novel differentiation intermediates of the ectoderm lineage

Citation

Harvey, NT and Hughes, JN and Lonic, A and Yap, C and Long, C and Rathjen, PD and Rathjen, J, Response to BMP4 signalling during ES cell differentiation defines novel differentiation intermediates of the ectoderm lineage, Journal of Cell Science, 123 pp. 1796-1804. ISSN 0021-9533 (2010) [Refereed Article]

DOI: doi:10.1242/jcs.047530

Abstract

The formation and differentiation of multipotent precursors underlies the generation of cell diversity during mammalian development. Recognition and analysis of these transient cell populations has been hampered by technical difficulties in accessing them in vivo. In vitro model systems, based on the differentiation of embryonic stem (ES) cells, provide an alternative means of identifying and characterizing these populations. Using a previously established mouse ES-cell-based system that recapitulates the development of the ectoderm lineage we have identified a transient population that is consistent with definitive ectoderm. This previously unidentified progenitor occurs as a temporally discrete population during ES cell differentiation, and differs from the preceding and succeeding populations in gene expression and differentiation potential, with the unique ability to form surface ectoderm in response to BMP4 signalling.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Cell Development, Proliferation and Death
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Rathjen, PD (Professor Peter Rathjen)
Author:Rathjen, J (Dr Joy Rathjen)
ID Code:78724
Year Published:2010
Web of Science® Times Cited:13
Deposited By:Menzies Institute for Medical Research
Deposited On:2012-07-24
Last Modified:2012-07-24
Downloads:0

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