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Response to BMP4 signalling during ES cell differentiation defines novel differentiation intermediates of the ectoderm lineage
journal contribution
posted on 2023-05-17, 12:36 authored by Harvey, NT, Hughes, JN, Lonic, A, Yap, C, Long, C, Rathjen, PD, Rathjen, JThe formation and differentiation of multipotent precursors underlies the generation of cell diversity during mammalian development. Recognition and analysis of these transient cell populations has been hampered by technical difficulties in accessing them in vivo. In vitro model systems, based on the differentiation of embryonic stem (ES) cells, provide an alternative means of identifying and characterizing these populations. Using a previously established mouse ES-cell-based system that recapitulates the development of the ectoderm lineage we have identified a transient population that is consistent with definitive ectoderm. This previously unidentified progenitor occurs as a temporally discrete population during ES cell differentiation, and differs from the preceding and succeeding populations in gene expression and differentiation potential, with the unique ability to form surface ectoderm in response to BMP4 signalling.
History
Publication title
Journal of Cell ScienceVolume
123Pagination
1796-1804ISSN
0021-9533Department/School
Menzies Institute for Medical ResearchPublisher
Company Of Biologists LtdPlace of publication
Bidder Building Cambridge Commercial Park Cowley Rd, Cambridge, England, Cambs, Cb4 4DlRepository Status
- Restricted