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Preliminary success using hydrogen peroxide to treat Atlantic salmon Salmo salar L. affected with experimentally induced amoebic gill disease (AGD)

Citation

Adams, MB and Crosbie, PBB and Nowak, BF, Preliminary success using hydrogen peroxide to treat Atlantic salmon Salmo salar L. affected with experimentally induced amoebic gill disease (AGD), Journal of Fish Diseases, 35, (11) pp. 839 -848. ISSN 0140-7775 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 Blackwell Publishing Ltd

DOI: doi:10.1111/j.1365-2761.2012.01422.x

Abstract

Currently the only effective and commercially used treatment for amoebic gill disease (AGD) in farmed Tasmanian Atlantic salmon is freshwater bathing. Hydrogen peroxide (H2O2), commonly used throughout the aquaculture industry for a range of topical skin and gill infections, was trialled in vitro and in vivo to ascertain its potential as an alternative treatment against AGD. Under in vitro conditions, trophozoites of Neoparamoeba perurans were exposed to three concentrations of H2O2 in seawater (500, 1000 & 1500 mg.l-1) over four durations (10, 20, 30 & 60 min) each at two temperatures (12 & 18C). Trophozoite viability was assessed immediately post exposure and after 24 hours. A concentration/duration combination of 1000 mg.l-1 for > 10 min demonstrated potent amoebicidal activity. Subsequently, Atlantic salmon mildly affected with experimentally induced AGD were treated with H2O2 at 12 and 18C for 15 minutes at 1250 mg.l-1 and their re-infection rate compared to freshwater treated fish over 21 days. Significant differences in the percentage of filaments affected with hyperplastic lesions (in association with amoebae) and plasma osmolality were noted between treatment groups immediately post-bath. However, the results were largely equivocal in terms of disease resolution over a three week period following treatment. These data suggest that H2O2 treatment in seawater successfully ameliorated a clinically light case of AGD under laboratory conditions.

Item Details

Item Type:Refereed Article
Keywords:AGD, Neoparamoeba, gills, parasite, hyperplasia, treatment
Research Division:Agricultural and Veterinary Sciences
Research Group:Veterinary Sciences
Research Field:Veterinary Anaesthesiology and Intensive Care
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Crustaceans (excl. Rock Lobster and Prawns)
Author:Adams, MB (Dr Mark Adams)
Author:Crosbie, PBB (Dr Philip Crosbie)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:78592
Year Published:2012
Web of Science® Times Cited:26
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2012-07-09
Last Modified:2013-05-24
Downloads:0

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