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Linking oxidative and salinity stress tolerance in barley: can root antioxidant enzyme activity be used as a measure of stress tolerance?
Citation
Maksimovic, JD and Zhang, J and Zeng, F and Ziavanovic, BD and Shabala, L and Zhou, M and Shabala, S, Linking oxidative and salinity stress tolerance in barley: can root antioxidant enzyme activity be used as a measure of stress tolerance?, Plant and Soil, 365, (1-2) pp. 141-155. ISSN 1573-5036 (2013) [Refereed Article]
Copyright Statement
Copyright Springer Science+Business Media B.V. 2012
DOI: doi:10.1007/s11104-012-1366-5
Abstract
Aims A causal relationship between salinity and oxidative stress tolerance and a suitability of using root antioxidant activity as a biochemical marker for salinity tolerance in barley was investigated.
Methods Net ion fluxes were measured from the mature zone of excised roots of two barle varieties contrasting in their salinity tolerance using non-invasive MIFE technique in response to acute and prolonged salinity treatment. These changes were correlated with activity of major antioxidant enzymes; ascorbate peroxidase, catalase, and superoxide dismutase.
Results It was found that genotypic difference in salinity tolerance was largely independent of root integrity, and observed not only for short-term but also long-term NaCl exposures. Higher K+ retention ability (and, hence, salinity tolerance) positively correlated with oxidative stress tolerance. At the same time, antioxidant activities were constitutively higher in a sensitive but not tolerant variety, and no correlation was found between SOD activity and salinity tolerance index during large-scale screening.
Conclusion although salinity tolerance in barley correlates with its oxidative stress tolerance, higher antioxidant activity at one particular time does not correlate with salinity tolerance and, as such, cannot be used as a biochemical marker in barley screening programs.
Methods Net ion fluxes were measured from the mature zone of excised roots of two barle varieties contrasting in their salinity tolerance using non-invasive MIFE technique in response to acute and prolonged salinity treatment. These changes were correlated with activity of major antioxidant enzymes; ascorbate peroxidase, catalase, and superoxide dismutase.
Results It was found that genotypic difference in salinity tolerance was largely independent of root integrity, and observed not only for short-term but also long-term NaCl exposures. Higher K+ retention ability (and, hence, salinity tolerance) positively correlated with oxidative stress tolerance. At the same time, antioxidant activities were constitutively higher in a sensitive but not tolerant variety, and no correlation was found between SOD activity and salinity tolerance index during large-scale screening.
Conclusion although salinity tolerance in barley correlates with its oxidative stress tolerance, higher antioxidant activity at one particular time does not correlate with salinity tolerance and, as such, cannot be used as a biochemical marker in barley screening programs.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | oxidative stress, ascorbate peroxidase, superoxide dismutase, root, ion flux, potassium homeostasis, hydrogen peroxide, superoxide anion radical, hydroxyl radical |
| Research Division: | Agricultural and Veterinary Sciences |
| Research Group: | Agriculture, Land and Farm Management |
| Research Field: | Sustainable Agricultural Development |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Biological Sciences |
| Author: | Zhang, J (Miss Jingyi Zhang) |
| Author: | Ziavanovic, BD (Dr Branka Zivanovic) |
| Author: | Shabala, L (Dr Lana Shabala) |
| Author: | Zhou, M (Professor Meixue Zhou) |
| Author: | Shabala, S (Professor Sergey Shabala) |
| ID Code: | 78591 |
| Year Published: | 2013 |
| Web of Science® Times Cited: | 26 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2012-07-09 |
| Last Modified: | 2017-11-06 |
| Downloads: | 0 |
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