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Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs
Citation
Earp, A and Hanson, CE and Ralph, PJ and Brando, VE and Allen, SS and Baird, M and Clementson, L and Daniel, P and Dekker, AG and Fearns, PRCS and Parslow, J and Strutton, PG and Thompson, PA and Underwood, M and Weeks, S and Doblin, MA, Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs, Optics Express, 19, (27) pp. 26768-26782. ISSN 1094-4087 (2011) [Refereed Article]
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Copyright Statement
Copyright 2011 Optical Society of America
Abstract
Fluorometers are widely used in ecosystem observing to monitor
fluorescence signals from organic compounds, as well as to infer
geophysical parameters such as chlorophyll or CDOM concentration, but
measurements are susceptible to variation caused by biofouling, instrument
design, sensor drift, operating environment, and calibration rigor. To collect
high quality data, such sensors need frequent checking and regular
calibration. In this study, a wide variety of both liquid and solid fluorescent
materials were trialed to assess their suitability as reference standards for
performance assessment of in situ fluorometers. Criteria used to evaluate
the standards included the spectral excitation/emission responses of the
materials relative to fluorescence sensors and to targeted ocean properties,
the linearity of the fluorometer’s optical response with increasing
concentration, stability and consistency, availability and ease of use, as well
as cost. Findings are summarized as a series of recommended reference
standards for sensors deployed on stationary and mobile platforms, to suit a
variety of in situ coastal to ocean sensor configurations. Repeated
determinations of chlorophyll scale factor using the recommended liquid
standard, Fluorescein, achieved an accuracy of 2.5%. Repeated
measurements with the recommended solid standard, Plexiglas Satinice®
plum 4H01 DC (polymethylmethacrylate), over an 18 day period varied
from the mean value by 1.0% for chlorophyll sensors and 3.3% for CDOM
sensors.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | fluorescent standards, reference standards, fluorometer, coastal and ocean observing program, chlorophyll |
| Research Division: | Physical Sciences |
| Research Group: | Atomic, molecular and optical physics |
| Research Field: | Atomic, molecular and optical physics not elsewhere classified |
| Objective Division: | Environmental Management |
| Objective Group: | Marine systems and management |
| Objective Field: | Measurement and assessment of marine water quality and condition |
| UTAS Author: | Allen, SS (Mr Simon Allen) |
| UTAS Author: | Strutton, PG (Professor Peter Strutton) |
| ID Code: | 78066 |
| Year Published: | 2011 |
| Web of Science® Times Cited: | 24 |
| Deposited By: | IMAS Research and Education Centre |
| Deposited On: | 2012-06-13 |
| Last Modified: | 2017-11-01 |
| Downloads: | 0 |
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