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Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs

Citation

Earp, A and Hanson, CE and Ralph, PJ and Brando, VE and Allen, SS and Baird, M and Clementson, L and Daniel, P and Dekker, AG and Fearns, PRCS and Parslow, J and Strutton, PG and Thompson, PA and Underwood, M and Weeks, S and Doblin, MA, Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs, Optics Express, 19, (27) pp. 26768-26782. ISSN 1094-4087 (2011) [Refereed Article]


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Copyright Statement

Copyright 2011 Optical Society of America

DOI: doi:10.1364/OE.19.026768

Abstract

Fluorometers are widely used in ecosystem observing to monitor fluorescence signals from organic compounds, as well as to infer geophysical parameters such as chlorophyll or CDOM concentration, but measurements are susceptible to variation caused by biofouling, instrument design, sensor drift, operating environment, and calibration rigor. To collect high quality data, such sensors need frequent checking and regular calibration. In this study, a wide variety of both liquid and solid fluorescent materials were trialed to assess their suitability as reference standards for performance assessment of in situ fluorometers. Criteria used to evaluate the standards included the spectral excitation/emission responses of the materials relative to fluorescence sensors and to targeted ocean properties, the linearity of the fluorometer’s optical response with increasing concentration, stability and consistency, availability and ease of use, as well as cost. Findings are summarized as a series of recommended reference standards for sensors deployed on stationary and mobile platforms, to suit a variety of in situ coastal to ocean sensor configurations. Repeated determinations of chlorophyll scale factor using the recommended liquid standard, Fluorescein, achieved an accuracy of 2.5%. Repeated measurements with the recommended solid standard, Plexiglas Satinice® plum 4H01 DC (polymethylmethacrylate), over an 18 day period varied from the mean value by 1.0% for chlorophyll sensors and 3.3% for CDOM sensors.

Item Details

Item Type:Refereed Article
Keywords:fluorescent standards, reference standards, fluorometer, coastal and ocean observing program, chlorophyll
Research Division:Physical Sciences
Research Group:Optical Physics
Research Field:Optical Physics not elsewhere classified
Objective Division:Environment
Objective Group:Physical and Chemical Conditions of Water
Objective Field:Physical and Chemical Conditions of Water in Marine Environments
Author:Allen, SS (Mr Simon Allen)
Author:Strutton, PG (Associate Professor Peter Strutton)
ID Code:78066
Year Published:2011
Web of Science® Times Cited:10
Deposited By:IMAS Research and Education Centre
Deposited On:2012-06-13
Last Modified:2012-10-29
Downloads:0

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