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Base-induced transient isotachophoretic stacking of acidic drugs in capillary zone electrophoresis


Quirino, JP, Base-induced transient isotachophoretic stacking of acidic drugs in capillary zone electrophoresis, Journal of Separation Science, 34, (9) pp. 1020-1026. ISSN 1615-9306 (2011) [Refereed Article]

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Copyright 2011 Wiley-VCH Verlag GmbH

DOI: doi:10.1002/jssc.201000748


Online sample concentration of acidic drugs by transient isotachophoresis (t-ITP) with the injection of a base is described in capillary zone electrophoresis (CZE). A positively coated capillary was conditioned with background electrolyte (ammonium acetate at pH 6). A long plug of sample solution (S) prepared in ammonium acetate was then hydrodynamically injected followed by the base (tetrapropylammonium hydroxide). A negative voltage was applied and caused the hydroxide ions from the base to penetrate the S zone and created a pH junction that swept through the S zone. The analytes stack at the junction where the mechanism of focusing was transient ITP with the acetate and hydroxide ions as leading and terminating ions, respectively. The concentrated analytes separated in co-EOF CZE once the hydroxide was exhausted. The base stacking strategy was tested using hypolipidemic, nonsteroidal anti-inflammatory, and diuretic drugs, and afforded 1937 improvements in peak height.

Item Details

Item Type:Refereed Article
Keywords:Base stacking; Capillary zone electrophoresis; Acidic drugs; Online sample concentration; Transient isotachophoresis
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:78056
Year Published:2011
Web of Science® Times Cited:17
Deposited By:Chemistry
Deposited On:2012-06-13
Last Modified:2017-10-31

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