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The neuronal p35 activator of Cdk5 is a novel F-actin binding and bundling protein

Citation

He, LS and Zhang, ZJ and Yu, Y and Ahmed, S and Cheung, NS and Qi, RZ, The neuronal p35 activator of Cdk5 is a novel F-actin binding and bundling protein, Cellular and Molecular Life Sciences, 68, (6) pp. 1633-1643. ISSN 1420-682X (2011) [Refereed Article]


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Copyright Statement

Copyright 2011 Springer Basel AG

DOI: doi:10.1007/s00018-010-0562-9

Abstract

The neuronal Cdk5 activator p35 is involved in a multitude of neuronal activities, including cytoskeletal organization. We show here that p35 directly interacts with filamentous actin (F-actin) but not with monomeric actin (G-actin). Through binding, p35 induces the formation of actin bundles and stabilizes F-actin against dilution-induced depolymerization. p35 forms intermolecular self-associations, suggesting that p35 cross-links actin filaments into bundles via its intermolecular self-association. p35 dimerization and association with F-actin occur at the N-terminal region that is absent in the calpain-cleaved product p25, indicating that such p35 properties are lost by its truncation induced under neurotoxic conditions. Using p35 phosphorylated by Cdk5 and a mutational approach, we demonstrate that the phosphorylation of p35 promotes its homodimerization and p35-induced formation of F-actin bundles. In addition, the phosphorylation regulates p35 distribution to microtubule and actin cytoskeletons. Together, these observations define a novel function for p35 in cytoskeletal regulation.

Item Details

Item Type:Refereed Article
Keywords:Cdk5, Cdk5 activator,  Actin, Actin-binding proteins, Protein phosphorylation
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Cell Neurochemistry
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:Cheung, NS (Dr Nam Cheung)
ID Code:77847
Year Published:2011
Web of Science® Times Cited:10
Deposited By:Menzies Institute for Medical Research
Deposited On:2012-06-01
Last Modified:2012-08-30
Downloads:0

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