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PCR screening for beta-lactamase-negative ampicillin-resistant strains of Haemophilus influenzae

Citation

Tristram, SG and Witherden, E, PCR screening for beta-lactamase-negative ampicillin-resistant strains of Haemophilus influenzae, Proceedings of European Congress on Clinical Microbiology and Infectious Diseases, March-April 2012, London, pp. 1. (2012) [Conference Extract]

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Abstract

Problems with MIC based methods and breakpoints, has prompted a move to genotypic definition and detection of B-lactamase negative ampicillin resistant (BLNAR) H. influenzae. Almost all BLNAR strains have an N526K substitution in penicillin binding protein 3 (PBP3) and single nucleotide specific (snp) PCR methods have been developed to interrogate the ftsI gene relevant to that substitution. In published methods, the design of specific reverse primers was based on the AAT (N) to AAG (K) mutation at the 1576-1578 codon seen in BLNAR strains sequenced at that time. There is relatively little published data on the evaluation of these primers, and their performance is in question as it is now recognised that in many BLNAR strains the N526K is associated with AAA. This study aims to evaluate existing and new snp PCR primers for detecting N526K BLNAR H. influenzae.

Item Details

Item Type:Conference Extract
Keywords:BLNAR H. influenzae
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Bacteriology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Tristram, SG (Dr Stephen Tristram)
Author:Witherden, E (Ms Elizabeth Witherden)
ID Code:77824
Year Published:2012
Deposited By:Health Sciences A
Deposited On:2012-05-30
Last Modified:2012-06-01
Downloads:0

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