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Description of an Atlantic salmon (Salmo salar L.) type ΙΙ interleukin-1 receptor cDNA and analysis of interleukin-1 receptor expression on amoebic gill disease affected fish

Citation

Morrison, RN and Young, ND and Nowak, BF, Description of an Atlantic salmon (Salmo salar L.) type ΙΙ interleukin-1 receptor cDNA and analysis of interleukin-1 receptor expression on amoebic gill disease affected fish, Fish and Shellfish Immunology, 32, (6) pp. 1185-1190. ISSN 1050-4648 (2012) [Refereed Article]


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Copyright Statement

Copyright 2012 Elsevier Ltd.

DOI: doi:10.1016/j.fsi.2012.03.005

Abstract

Previously, we showed that IL-1b transcription is induced in the gills of amoebic gill disease (AGD)-affected fish in an AGD lesion-restricted fashion. However, in this environment, there is very little evidence of inflammation on histopathological or transcriptional levels and we hypothesised that aberrant signalling may occur. As a first step in investigating this issue, we cloned and sequenced the Atlantic salmon IL-1 receptor type II (IL-1RII) mRNA, and then examined the expression of both the IL-1RI (IL-1 receptor-like protein) and II during Neoparamoeba perurans infection. In gill lesions from AGD-affected fish, a step-wise temporal increase in the relative expression of IL-1β coincided with a significant reduction in IL-1RI, whereas the IL-1RII mRNA remained unchanged. Down-regulation of IL-1RI could explain the paucity of inflammation in affected tissue, although simultaneous up-regulation of IL-1β-inducible transcripts indicated that this is not due to a complete blockage of the IL-1RI pathway. Rather, it appears that IL-1RI transcription is reduced and this rate limits the effects of chronic IL-1β over-expression.

Item Details

Item Type:Refereed Article
Keywords:interleukin-1beta, IL-1 receptor, amoebic gill disease
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Morrison, RN (Dr Richard Morrison)
Author:Young, ND (Mr Neil Young)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:77809
Year Published:2012
Web of Science® Times Cited:16
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2012-05-30
Last Modified:2013-05-02
Downloads:0

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