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Pseudomonas siderophores in the sputum of patients with cystic fibrosis

Citation

Martin, LW and Reid, DW and Sharples, KJ and Lamont, IL, Pseudomonas siderophores in the sputum of patients with cystic fibrosis, Biometals, 24, (6) pp. 1059-1067. ISSN 0966-0844 (2011) [Refereed Article]


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Copyright 2011 Springer Science+Business Media, LLC.

DOI: doi:10.1007/s10534-011-9464-z

Abstract

The lungs of patients with cystic fibrosis become chronically infected with the bacterium Pseudomonas aeruginosa, which heralds progressive lung damage and a decline in health. Iron is a crucial micronutrient for bacteria and its acquisition is a key factor in infection. P. aeruginosa can acquire this element by secreting pyoverdine and pyochelin, ironchelating compounds (siderophores) that scavenge iron and deliver it to the bacteria. Siderophoremediated iron uptake is generally considered a key factor in the ability of P. aeruginosa to cause infection. We have investigated the amounts of pyoverdine in 148 sputum samples from 36 cystic fibrosis patients (30 infected with P. aeruginosa and 6 as negative controls). Pyoverdine was present in 93 samples in concentrations between 0.30 and 51 lM (median 4.6 lM) and there was a strong association between the amount of pyoverdine and the number of P. aeruginosa present. However, pyoverdine was not present, or below the limits of detection (*0.3 lM), in 21 sputum samples that contained P. aeruginosa. Pyochelin was also absent, or below the limits of detection (*1 lM), in samples from P. aeruginosainfected patients with little or no detectable pyoverdine. Our data show that pyoverdine is an important iron-scavenging molecule for P. aeruginosa in many cystic fibrosis patients, but other P. aeruginosa iron uptake systems must be active in some patients to satisfy the bacterial need for iron.

Item Details

Item Type:Refereed Article
Keywords:Pyoverdine,  Pyochelin,  Iron and infection, Pseudomonas aeruginosa,  Cystic fibrosis
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Bacteriology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Respiratory System and Diseases (incl. Asthma)
Author:Reid, DW (Dr David Reid)
ID Code:77807
Year Published:2011
Web of Science® Times Cited:30
Deposited By:Menzies Institute for Medical Research
Deposited On:2012-05-30
Last Modified:2012-08-30
Downloads:0

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