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Comparison of passive and active immunization of fish against streptococcosis (enterococcosis)

Citation

Akhlaghi, M and Munday, BL and Whittington, RJ, Comparison of passive and active immunization of fish against streptococcosis (enterococcosis), Journal of Fish Diseases, 19, (3) pp. 251-258. ISSN 0140-7775 (1996) [Refereed Article]

DOI: doi:10.1111/j.1365-2761.1996.tb00132.x

Abstract

Passive immunization of rainbow trout, Oncorhynchus mykiss (Walbaum), was carried out to determine the persistence of anti-Streptococcus sp. antibodies (ASA) raised in sheep, rabbits or rainbow trout. The protection afforded by passive immunization was compared with the protection obtained from active immunization by immersion in or intraperitoneal (i.p.) injection with formalin-killed cells. Assessments were undertaken concurrently for up to 3 months post-immunization (PI) to evaluate the practical potential of passive immunization. Passively administered sheep and rabbit antibodies were detected in fish sera by enzyme-linked immunosorbent assay for more than 60 days after i.p. injection. Fish responded immunologically to these antibodies and the highest humoral responses to sheep and rabbit ASA occurred at 2 months PI. The relative per cent survival (RPS) of rainbow trout challenged with virulent Streptococcus sp. after an i.p. injection (0-1 ml 100 g-1 fish body weight) of sheep, rabbit or fish ASA was: 88·8, 50 and 0% after 1 month; 33·3, 6·8 and 6·8% after 2 months; and 13·3, O and 6·6% after 3 months PI, respectively. Fish immunized actively had an RPS of 88·8 and 11·1% after 1 month, 38·1 and 4·7% after 2 months, and 36 and 0% after 3 months PI for the i.p. injection and immersion routes, respectively.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Wild Caught
Objective Field:Fisheries - Wild Caught not elsewhere classified
Author:Akhlaghi, M (Mr Mostafa Akhlaghi)
Author:Munday, BL (Dr Barry Munday)
ID Code:7780
Year Published:1996
Web of Science® Times Cited:26
Deposited By:TAFI - Aquaculture
Deposited On:1996-08-01
Last Modified:2011-08-19
Downloads:0

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