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Simultaneous Electrokinetic and Hydrodynamic Injection for High Sensitivity Bacteria Analysis in Capillary Electrophoresis

Citation

Oukacine, F and Quirino, JP and Garrelly, L and Romestand, B and Zou, T and Cottet, H, Simultaneous Electrokinetic and Hydrodynamic Injection for High Sensitivity Bacteria Analysis in Capillary Electrophoresis, Analytical Chemistry, 83, (12) pp. 4949-4954. ISSN 0003-2700 (2011) [Refereed Article]


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Copyright Statement

Copyright 2011 American Chemical Society

DOI: doi:10.1021/ac200684t

Abstract

A repeatable preconcentration electrophoretic methodology for the analysis of bacteria was developed. This method is based on an isotachophoretic mode coupled with a simultaneous hydrodynamic-electrokinetic injection in conditions of field-amplified sample injection. This electrophoretic method allows the quantification of Enterobacter cloacae (studied as a model of Gram negative bacteria) with a limit of detection of 2  104 cells/mL. With the optimized conditions, a preconcentration factor of about 500-fold was obtained as compared to a standard hydrodynamic injection. The RSD (n = 5) on the migration time and on the peak area were 3% and 5%, respectively. This capillary electrophoretic methodology has been applied for the quantification of microbes in natural water (river and natural spring waters). Filtration of the sample prior to injection was required to remove ions present in the water and to keep the field-amplified sample injection condition at the injection. Filtrated bacteria were then recovered in terminating electrolyte diluted 10 times with water. Good agreements were obtained between cellular ATP measurements and the proposed CE methodology for the quantification of bacteria in waters.

Item Details

Item Type:Refereed Article
Keywords:repeatable preconcentration electrophoretic methodology, bacteria, isotachophoretic mode, hydrodynamic-electrokinetic injection, field-amplified sample injection, Enterobacter cloacae, Gram negative bacteria, standard hydrodynamic injection, microbes
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:77787
Year Published:2011
Web of Science® Times Cited:24
Deposited By:Chemistry
Deposited On:2012-05-29
Last Modified:2012-08-27
Downloads:0

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