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Preliminary investigation onto the killing effect of kingfish (Seliola lalandi) serum and mucus against the monogenean parasites Benedenia seriolae and Zeuxapta seriolae
journal contribution
posted on 2023-05-17, 11:44 authored by Melanie Leef, Lee, PSAbstract The survival of two economically important monogenean parasites, Benedenia seriolae and Zeuxapta seriolae, exposed to either nayve or infected yellowtail kingfish Seriola lalandi serum and mucus was observed over an 8-h period. Infection status of the host had no effect on survival of monogeneans in either serum or mucus therefore data were grouped according to each parasite. Serum exposure at the highest concentration of 1:5 did not have any effect on Z. seriolae, however, 50% killing activity against B. seriolae was observed within 3 h at concentrations greater than 1:100. This killing activity was completely abolished following heat treatment and inactivation of the serum. Prior incubation of parasites with heat-treated serum for 2 h in order to potentially coat the parasites with antibodies did not enhance the activity of fresh serum against either species. Addition of 5 mM EDTA, but not 5 mM EGTA-Mg, inhibited the killing ability of fresh serum against B. seriolae, suggesting that previously observed activity against this parasite was most likely mediated via the alternative complement pathway. Cutaneous mucus was not found to have any effect on B. seriolae or Z. seriolae survival. Overall these results suggest that the blood-feeding gill parasite Z. seriolae is considerably more resistant to host immune responses compared with skin- and mucus-dwelling B. seriolae. Keywords Yellowtail kingfish Seriola lalandi Monogenean Innate immunity Benedenia seriolae Zeuxapta seriolae Classical and alternative complement activity Parasitic disease Aquaculture M. J. Leef (&) P. S. Lee National Institute of Water and Atmospheric Research Ltd., Bream Bay Aquaculture, PO Box 147, Ruakaka, New Zealand e-mail: mleef@utas.edu.au Present Address:
History
Publication title
Aquaculture: An International Journal Devoted to Fundamental Aquatic Food ResourcesIssue
17Pagination
607-614ISSN
0044-8486Department/School
Institute for Marine and Antarctic StudiesPublisher
Elsevier Science BvPlace of publication
Po Box 211, Amsterdam, Netherlands, 1000 AeRepository Status
- Restricted