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Preliminary investigation onto the killing effect of kingfish (Seliola lalandi) serum and mucus against the monogenean parasites Benedenia seriolae and Zeuxapta seriolae

Citation

Leef, MJ and Lee, PS, Preliminary investigation onto the killing effect of kingfish (Seliola lalandi) serum and mucus against the monogenean parasites Benedenia seriolae and Zeuxapta seriolae, Aquaculture: An International Journal Devoted to Fundamental Aquatic Food Resources, (17) pp. 607-614. ISSN 0044-8486 (2009) [Refereed Article]

DOI: doi:10.1007/s10499-008-9228-0

Abstract

Abstract The survival of two economically important monogenean parasites, Benedenia seriolae and Zeuxapta seriolae, exposed to either naż®ve or infected yellowtail kingfish Seriola lalandi serum and mucus was observed over an 8-h period. Infection status of the host had no effect on survival of monogeneans in either serum or mucus therefore data were grouped according to each parasite. Serum exposure at the highest concentration of 1:5 did not have any effect on Z. seriolae, however, 50% killing activity against B. seriolae was observed within 3 h at concentrations greater than 1:100. This killing activity was completely abolished following heat treatment and inactivation of the serum. Prior incubation of parasites with heat-treated serum for 2 h in order to potentially coat the parasites with antibodies did not enhance the activity of fresh serum against either species. Addition of 5 mM EDTA, but not 5 mM EGTA-Mg, inhibited the killing ability of fresh serum against B. seriolae, suggesting that previously observed activity against this parasite was most likely mediated via the alternative complement pathway. Cutaneous mucus was not found to have any effect on B. seriolae or Z. seriolae survival. Overall these results suggest that the blood-feeding gill parasite Z. seriolae is considerably more resistant to host immune responses compared with skin- and mucus-dwelling B. seriolae. Keywords Yellowtail kingfish  Seriola lalandi  Monogenean  Innate immunity  Benedenia seriolae  Zeuxapta seriolae  Classical and alternative complement activity  Parasitic disease  Aquaculture M. J. Leef (&)  P. S. Lee National Institute of Water and Atmospheric Research Ltd., Bream Bay Aquaculture, PO Box 147, Ruakaka, New Zealand e-mail: mleef@utas.edu.au Present Address:

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Crustaceans (excl. Rock Lobster and Prawns)
Author:Leef, MJ (Dr Melanie Leef)
ID Code:77752
Year Published:2009
Web of Science® Times Cited:3
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2012-05-24
Last Modified:2012-10-01
Downloads:0

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