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Use of a blocking primer allows selective amplification of bacterial DNA from microalgae cultures

Citation

Powell, SM and Chapman, CC and Bermudes, M and Tamplin, ML, Use of a blocking primer allows selective amplification of bacterial DNA from microalgae cultures, Journal of Microbiological Methods, 90, (3) pp. 211-213. ISSN 0167-7012 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 Elsevier B.V.

DOI: doi:10.1016/j.mimet.2012.05.007

Abstract

The production of algae is a crucial component of many aquaculture systems and the role of bacteria in this process is an important although complex one. We report the development of a new blocking primer that allowed PCR amplification of bacterial DNA in the presence of algal chloroplast DNA.

Item Details

Item Type:Refereed Article
Keywords:blocking primer, microbial community, Isochrysis
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Powell, SM (Dr Shane Powell)
Author:Chapman, CC (Mr Chapman)
Author:Tamplin, ML (Professor Mark Tamplin)
ID Code:77647
Year Published:2012
Web of Science® Times Cited:2
Deposited By:Agricultural Science
Deposited On:2012-05-15
Last Modified:2013-06-12
Downloads:0

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