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Molecular analysis of the bacterial communities in the live Pacific oyster (Crassostrea gigas) and the influence of postharvest temperature on its structure

Citation

Fernandez Piquer, J and Bowman, JP and Ross, T and Tamplin, ML, Molecular analysis of the bacterial communities in the live Pacific oyster (Crassostrea gigas) and the influence of postharvest temperature on its structure, Journal of Applied Microbiology, 112, (6) pp. 1134-1143. ISSN 1364-5072 (2012) [Refereed Article]


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Copyright Statement

Copyright 2012 The Society for Applied Microbiology

DOI: doi:10.1111/j.1365-2672.2012.05287.x

Abstract

Aims: To evaluate the effect of postharvest temperature on bacterial communities in live Pacific oysters (Crassostrea gigas) using nonculture-based methods.

Methods and Results: Live oysters were compared before and after storage at 4, 6, 15, 20 and 30C using terminal restriction fragment length polymorphism (T-RFLP). Bacterial communities in freshly harvested (control) vs stored oysters were significantly different. Changes in bacterial communities at 4, 15 and 30C observed by T-RFLP were further investigated by clone library analysis. Members of the Proteobacteria predominated (43.057.0% of clones) in control oysters, while storage altered the bacterial profile. At 4C, Psychrilyobacter spp.(phylum Fusobacteria) predominated (43.8% of clones), while at 15 and 30C, members of the phylum Bacteroidetes represented 63.0 and 60.2% of clones, respectively. High microbial diversity in oysters was observed, with at least 73 different genera-related clones among all samples.

Conclusions: Changes in the overall bacterial community of Pacific oysters were influenced by storage temperature and would likely not be detected by standard culture-based methods currently used to assess oyster quality. Certain dominant genera, such as Psychrilyobacter, Polynucleobacter and a bacterial group related to Alkaliflexus, should be further studied as possible indicators for postharvest temperature control.

Significance and Impact of the Study: This work is the first report describing the effect of different storage temperatures on bacterial diversity in postharvest live Pacific oysters using molecular-based methods.

Item Details

Item Type:Refereed Article
Keywords:16S rRNA, bacterial community analysis, clone library, Crassostrea gigas, Pacific oyster, T-RFLP
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Food Safety
Author:Fernandez Piquer, J (Dr Judith Fernandez-Piquer)
Author:Bowman, JP (Associate Professor John Bowman)
Author:Ross, T (Associate Professor Tom Ross)
Author:Tamplin, ML (Professor Mark Tamplin)
ID Code:77419
Year Published:2012
Web of Science® Times Cited:14
Deposited By:Agricultural Science
Deposited On:2012-04-12
Last Modified:2014-11-24
Downloads:7 View Download Statistics

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