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Development and validation of a predictive model for the growth of Vibrio parahaemolyticus in post-harvest shellstock oysters


Parveen, S and DaSilva, L and DePaola, A and Bowers, J and White, C and Munasinghe, KA and Brohawn, K and Mudoh, M and Tamplin, M, Development and validation of a predictive model for the growth of Vibrio parahaemolyticus in post-harvest shellstock oysters, International Journal of Food Microbiology, 161, (1) pp. 1-6. ISSN 0168-1605 (2013) [Refereed Article]

Copyright Statement

Copyright 2012 Elsevier B.V.

DOI: doi:10.1016/j.ijfoodmicro.2012.11.010


Information is limited about the growth and survival of naturally-occurring Vibrio parahaemolyticus in live oysters under commercially relevant storage conditions harvested from different regions and in different oyster species. This study produced a predictive model for the growth of naturally-occurring V. parahaemolyticus in live Eastern oysters (Crassostrea virginica) harvested from the Chesapeake Bay, MD, USA and stored at 530 C until oysters gapped. The model was validated with model-independent data collected from Eastern oysters harvested from the Chesapeake Bay and Mobile Bay, AL, USA and Asian (C. ariakensis) oysters from the Chesapeake Bay, VA, USA. The effect of harvest season, region and water condition on growth rate (GR) was also tested. At each time interval, two samples consisting of six oysters each were analyzed by a direct-plating method for total V. parahaemolyticus. The Baranyi D-model was fitted to the total V. parahaemolyticus growth and survival data. A secondary model was produced using the square root model. V. parahaemolyticus slowly inactivated at 5 and 10 C with average rates of −0.002 and −0.001 log cfu/h, respectively. The average GRs at 15, 20, 25, and 30 C were 0.038, 0.082, 0.228, and 0.219 log cfu/h, respectively. The bias and accuracy factors of the secondary model for model-independent data were 1.36 and 1.46 for Eastern oysters from Mobile Bay and the Chesapeake Bay, respectively. V. parahaemolyticus GRs were markedly lower in Asian oysters. Harvest temperature, salinity, region and season had no effect on GRs. The observed GRs were less than those predicted by the U.S. Food and Drug Administration's V. parahaemolyticus quantitative risk assessment.

Item Details

Item Type:Refereed Article
Keywords:Vibrio parahaemolyticus, predictive microbiology, secondary mode, Eastern oyster, Asian oyster
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Health
Objective Group:Public health (excl. specific population health)
Objective Field:Food safety
UTAS Author:Tamplin, M (Professor Mark Tamplin)
ID Code:77413
Year Published:2013
Web of Science® Times Cited:33
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2012-04-11
Last Modified:2014-04-03

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