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Online sample pre-concentration via dynamic pH junction in capillary and microchip electrophoresis


Kazarian, AA and Hilder, EF and Breadmore, MC, Online sample pre-concentration via dynamic pH junction in capillary and microchip electrophoresis, Journal of Separation Science, 34, (20) pp. 2800-2821. ISSN 1615-9314 (2011) [Refereed Article]

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Copyright 2011 WILEY-VCH Verlag GmbH & Co. KGaA

DOI: doi:10.1002/jssc.201100414


Various analytical techniques have been developed over the years to analyse a large diversity of biomolecules with a constant push towards ultra-sensitive detection. CE is at the forefront of the most powerful analytical tools available to date when considering its superior efficiency and resolution; however, the technique suffers from poor sensitivity as a result of the short path length at the detection site and small injection volumes (typically o1% capillary length). One of the approaches to abate the inherent problem is to employ clever chemistry using sample focusing techniques whereby a large sample plug can be injected, preconcentrated and separated, producing excellent sensitivity and efficiency at the detector. This particular review will focus on the use of dynamic pH junction as a means of improving sensitivity in CE and focuses on the use of a change in analyte ionisation due to different pHs between the sample and electrolyte. The review provides a fundamental discussion of the mechanisms, buffer and sample conditions required to concentrate various analytes and a comprehensive list of published works in tabular format for easy identification of suitable conditions for new applications. The review further encompasses the use of dynamic pH junction in CE and its involvement in combination with other preconcentrations techniques to produce high sensitivity enhancements recorded between the years 19902010.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Kazarian, AA (Dr Tom Kazarian)
UTAS Author:Hilder, EF (Professor Emily Hilder)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:76840
Year Published:2011
Web of Science® Times Cited:49
Deposited By:Chemistry
Deposited On:2012-03-14
Last Modified:2017-10-30

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