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Separation and sweeping of metal ions with EDTA in CZE-ESI-MS


Quirino, JP and Haddad, PR, Separation and sweeping of metal ions with EDTA in CZE-ESI-MS, Journal of Separation Science, 34, (20) pp. 2872-2878. ISSN 1615-9314 (2011) [Refereed Article]

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Copyright 2011 WILEY-VCH Verlag GmbH & Co. KGaA

DOI: doi:10.1002/jssc.201100290


Ethylenediaminetetraacetic acid (EDTA) in the background electrolyte (BGE) of capillary zone electrophoresis coupled to an electrospray ionization mass spectrometer is presented as an approach for the determination of metal ions. Significant signals for the metal–ligand complexes were observed even when EDTA was continually eluted from the capillary during the entire electrophoretic run. The signal-to-noise ratio was improved by the addition of ammonia to the sheath liquid and by using an acquisition m/z range above the m/z of EDTA. The LODs for the test metal ions (i.e. calcium(II), manganese(II), and zinc(II)) with conventional injection were around 1–2 mg/L with corrected peak areas that are linear from 8 to 100 mg/L. The presence of EDTA in the BGE was critical not only for the separation but also for sweeping via complexation as an on-line sample concentration technique. The peak height of the test metal ions was improved at least tenfold with sweeping via EDTA complexation and yielded LODs in the mg/L range.

Item Details

Item Type:Refereed Article
Keywords:Capillary zone electrophoresis, Electrospray ionization mass, Ethylenediaminetetraacetic acid, Metal ions, Sweeping
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Analytical spectrometry
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:76832
Year Published:2011
Web of Science® Times Cited:12
Deposited By:Chemistry
Deposited On:2012-03-14
Last Modified:2012-07-05

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