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Kinetic optimisation of open-tubular liquid-chromatography capillaries coated with thick porous layers for increased loadability

journal contribution
posted on 2023-05-17, 11:10 authored by Causon, TJ, Robert ShellieRobert Shellie, Emily HilderEmily Hilder, Desmet, G, Eeltink, S
The kinetic optimisation of open-tubular liquid chromatography (OTLC) columns has been revisited by taking the thick-film effects for porous coatings on retention, column resistance, band broadening and mass loadability into account. Considering the most advantageous case (i.e. where the retentive layer allows for the same high internal diffusion coefficient as conventional porous particles), calculations show the need for the development of coating procedures leading to porous films filling up approximately 50-70% of the total column diameter. Furthermore, to achieve optimum kinetic performance for separations of small molecules with total analysis times of less than 8 h (k = 9), total column diameters should be less than 6 um with lengths typically greater than 0.8 m for N values of 125,000-500,000 at a pressure of 400 bar. The use of elevated temperature LC (90 Celsius degree) is also shown to increase the allowable total column diameter to up to 9 um for a larger range of N values (100,000-880,000).

Funding

Australian Research Council

History

Publication title

Journal of Chromatography A

Volume

1218

Issue

46

Pagination

8388-8393

ISSN

0021-9673

Department/School

School of Natural Sciences

Publisher

Elsevier Science Bv

Place of publication

PO Box 211, Amsterdam, Netherlands, 1000 Ae

Rights statement

The definitive version is available at http://www.sciencedirect.com

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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