University of Tasmania
Browse

File(s) under permanent embargo

Fast and sensitive determination of aluminium with RP-HPLC using an ultrashort monolithic column

journal contribution
posted on 2023-05-17, 11:08 authored by Tomas Remenyi, Nesterenko, PN, Andrew BowieAndrew Bowie, Butler, ECV, Paul HaddadPaul Haddad
A fast and sensitive reversed-phase high-performance liquid-chromatographic method for determination of aluminium in aqueous samples has been developed. The fluorescent aluminiumlumogallion complex (lex 505 nm, lem 574 nm), was formed with a pre-column reagent and then separated on a Chromolith RP-18e Guard column using a two-tiered, stepped gradient program, with matrix elimination (5/95 (v/v) methanol/water), followed by elution (90/10 (v/v) methanol/water). This method achieved a run time of 2.5 min without compromising sensitivity (limit of detection = 2.5 x 10 -7 M, limit of quantification = 7.2 x 10 -7 M), precision (3.5% at 3.71 x 10 -6 M) or accuracy (recovery = 97.5% +- 3.2%, n = 5, P = 0.95). This represents an improvement in run time by >50% compared to the fastest previously published method [Lee et al., Clin. Chem., 1996, 42, 1405-1411]. This method uses MES buffer (2-(N-morpholino)ethanesulfonic acid), which compared to other buffers is more easily purified, does not complex with aluminium, and can be used in lower concentrations. This method was applied to analysis of: deionised water for column comparison; seawater for matrix interference effects; and tea-infusion for calibration and recovery studies.

History

Publication title

Analytical Methods

Issue

11

Pagination

2488-2494

ISSN

1759-9660

Department/School

Institute for Marine and Antarctic Studies

Publisher

The Royal Society of Chemistry

Place of publication

Cambridge, UK

Rights statement

Copyright 2011 The Royal Society of Chemistry

Repository Status

  • Restricted

Socio-economic Objectives

Antarctic and Southern Ocean oceanic processes

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC