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Fast and sensitive determination of aluminium with RP-HPLC using an ultrashort monolithic column


Remenyi, TA and Nesterenko, PN and Bowie, AR and Butler, ECV and Haddad, PR, Fast and sensitive determination of aluminium with RP-HPLC using an ultrashort monolithic column, Analytical Methods, 3, (11) pp. 2488-2494. ISSN 1759-9660 (2011) [Refereed Article]

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Copyright Statement

Copyright 2011 The Royal Society of Chemistry

DOI: doi:10.1039/c1ay05192j


A fast and sensitive reversed-phase high-performance liquid-chromatographic method for determination of aluminium in aqueous samples has been developed. The fluorescent aluminiumlumogallion complex (lex 505 nm, lem 574 nm), was formed with a pre-column reagent and then separated on a Chromolith RP-18e Guard column using a two-tiered, stepped gradient program, with matrix elimination (5/95 (v/v) methanol/water), followed by elution (90/10 (v/v) methanol/water). This method achieved a run time of 2.5 min without compromising sensitivity (limit of detection 2.5  107 M, limit of quantification 7.2  107 M), precision (3.5% at 3.71  106 M) or accuracy (recovery 97.5%  3.2%, n 5, P 0.95). This represents an improvement in run time by >50% compared to the fastest previously published method [Lee et al., Clin. Chem., 1996, 42, 14051411]. This method uses MES buffer (2-(N-morpholino)ethanesulfonic acid), which compared to other buffers is more easily purified, does not complex with aluminium, and can be used in lower concentrations. This method was applied to analysis of: deionised water for column comparison; seawater for matrix interference effects; and tea-infusion for calibration and recovery studies.

Item Details

Item Type:Refereed Article
Keywords:aluminium, ultra-short monolithic column, biogeochemistry
Research Division:Earth Sciences
Research Group:Oceanography
Research Field:Chemical oceanography
Objective Division:Environmental Management
Objective Group:Management of Antarctic and Southern Ocean environments
Objective Field:Antarctic and Southern Ocean oceanic processes
UTAS Author:Remenyi, TA (Dr Tom Remenyi)
UTAS Author:Nesterenko, PN (Professor Pavel Nesterenko)
UTAS Author:Bowie, AR (Professor Andrew Bowie)
UTAS Author:Butler, ECV (Dr Edward Butler)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:76796
Year Published:2011
Web of Science® Times Cited:2
Deposited By:CRC-Antarctic Climate & Ecosystems
Deposited On:2012-03-13
Last Modified:2017-10-26

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