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Porous Graphitized Carbon Monolith as an Electrode Material for Probing Direct Bioelectrochemistry and Selective Detection of Hydrogen Peroxide

Citation

He, X and Zhou, L and Nesterenko, EP and Nesterenko, PN and Paull, B and Omamogho, JO and Glennon, JD and Luong, JHT, Porous Graphitized Carbon Monolith as an Electrode Material for Probing Direct Bioelectrochemistry and Selective Detection of Hydrogen Peroxide, Analytical Chemistry, 84, (5) pp. 2351-2357. ISSN 0003-2700 (2012) [Refereed Article]


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Copyright Statement

Copyright 2012 American Chemical Society

DOI: doi:10.1021/ac203061m

Abstract

For the first time, graphitized carbon particles with a high surface area have been prepared and evaluated as a new material for probing direct electrochemistry of hemoglobin (Hb). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) imaging revealed that the carbon monolithic skeleton was constructed by a series of mesopores with irregular shapes and an average pore diameter of ´5.6 nm. With a surface area of 239.6 m2/g, carbon particles exhibited three major Raman peaks as commonly observed for carbon nanotubes and other carbon materials, i.e., the sp3 and sp2 carbon phases coexisted in the sample. A glassy carbon electrode modified with carbon monoliths and didodecyldimethylammonium bromide exhibited direct electron transfer between Hb molecules and the underlying electrode with a transfer rate constant of 6.87 s−1. The enzyme electrode displayed a pair of quasi-reversible reduction−oxidation peaks at −0.128 and −0.180 V, reflecting the well-known feature of the heme [Fe3+/Fe2+] redox couple: a surface-controlled electrochemical process with one electron transfer. This reagentless biosensing approach was capable of detecting H2O2, a simple molecule but plays an important role in analytical and biological chemistry, as low as 0.1 レM with linearity of 0.1−60 レM and a response time of <0.8 s, comparing favorably with other carbon based electrodes (5 s).

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Electroanalytical Chemistry
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Mathematical Sciences
Author:Nesterenko, PN (Professor Pavel Nesterenko)
Author:Paull, B (Professor Brett Paull)
ID Code:76751
Year Published:2012
Web of Science® Times Cited:37
Deposited By:Chemistry
Deposited On:2012-03-13
Last Modified:2017-10-26
Downloads:0

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