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Methods in neuronal growth cone biology

Citation

Gasperini, RJ and Foa, L, Methods in neuronal growth cone biology, TRP Channels in Drug Discovery. Methods in Pharmacology and Toxicology, Humana Press, T Biro & A Szallasi (ed), New York, pp. 239-252. ISBN 978-1-62703-094-6 (2012) [Research Book Chapter]

Copyright Statement

Copyright 2012 Springer Science+Business Media, LLC

DOI: doi:10.1007/978-1-62703-095-3

Abstract

Axon guidance is a crucial component of normal nervous system development. Errors in axon guidance can result in disorders such as autism and schizophrenia. In order to understand the pathogenesis of these disorders, we need to understand the fundamental mechanisms of growth cone motility, which ultimately direct axon guidance. In this chapter, we describe protocols and methods that our laboratory uses to study normal growth cone physiology and biochemistry. We describe the pipette turning assay [Lohof AM, Quillan M, Dan Y, Poo MM, J Neurosci 12(4): 1253-1261, 1992] that we have adapted for primary cultures of rodent dorsal root ganglia. We also describe methods for calcium imaging of growth cones and provide data to demonstrate the absolute requirement of TRP channels for growth cone navigation. These techniques provide researchers with considerable scope and flexibility in experimental design and can be adapted to provide high-throughput biochemical data for the elucidation of mechanisms governing the action of guidance molecules. © 2012 Springer Science+Business Media, LLC.

Item Details

Item Type:Research Book Chapter
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:Gasperini, RJ (Dr Rob Gasperini)
Author:Foa, L (Associate Professor Lisa Foa)
ID Code:76471
Year Published:2012
Deposited By:Medicine (Discipline)
Deposited On:2012-03-06
Last Modified:2017-10-16
Downloads:0

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