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Validation and quantification of extractable age pigments for determining the age of Antarctic krill (Euphausia superba)
journal contribution
posted on 2023-05-17, 10:53 authored by McGaffin, AF, Nicol, S, Patti VirtuePatti Virtue, Hirano, Y, Matsuda, T, Uchida, I, Candy, SG, Kawaguchi, SAntarctic krill, Euphausia superba, hatched from eggs and maintained for four years, were sampled periodically for age-pigment analysis. Extractable pigments from the eye and eyestalk ganglia were quantified using fluorescence intensity and standardised against protein. Three peak fluorescence intensities were detected at wavelengths of excitation 280 nm, emission 625 nm (pigment 1); excitation 355 nm, emission 510 nm (pigment 2); and excitation 463 nm, emission 620 nm (pigment 3). There was a positive correlation between the quantity of pigments 1 and 3 and the age of Antarctic krill. A model was developed to predict age from pigment 3 and to compare it with other age proxies (carapace length and eyeball diameter). The quantity of pigment 3 was the best predictor of age. The pigment method can discriminate between similar sized krill aged 12 and 36 months. Age pigments provide an improved tool for age estimation in Antarctic krill, particularly if used in conjunction with other demographic information.
History
Publication title
Marine Biology: International Journal on Life in Oceans and Coastal WatersVolume
158Issue
8Pagination
1743-1755ISSN
0025-3162Department/School
Institute for Marine and Antarctic StudiesPublisher
Springer-VerlagPlace of publication
175 Fifth Ave, New York, USA, Ny, 10010Rights statement
Copyright 2011 Springer-VerlagRepository Status
- Restricted