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Photosysnthetic Pigment and Genetic Differences Between Two Southern Ocean Morphotypes of Emiliania Huxleyi (Haptophyta)

Citation

Cook, SS and Whittock, LD and Wright, SW and Hallegraeff, GM, Photosysnthetic Pigment and Genetic Differences Between Two Southern Ocean Morphotypes of Emiliania Huxleyi (Haptophyta), Journal of Phycology, 47, (3) pp. 615-626. ISSN 0022-3646 (2011) [Refereed Article]


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The definitive published version is available online at: http://www3.interscience.wiley.com/

DOI: doi:10.1111/j.1529-8817.2011.00992.x

Abstract

The widespread coccolithophorid Emiliania huxleyi (Lohmann) W. W. Hay et H. Mohler plays a pivotal role in the carbon pump and is known to exhibit significant morphological, genetic, and physiological diversity. In this study, we compared photosynthetic pigments and morphology of triplicate strains of Southern Ocean types A and B ⁄ C. The two morphotypes differed in width of coccolith distal shield elements (0.11–0.24 lm, type A; 0.06–0.12 lm, type B ⁄ C) and morphology of distal shield central area (grill of curved rods in type A; thin plain plate in type B ⁄ C) and showed differences in carotenoid composition. The mean 19¢-hexanoyloxyfucoxanthin (Hex):chl a ratio in type B ⁄C was >1, whereas the type A ratio was <1. The Hex:fucoxanthin (fuc) ratio for type B ⁄C was 11 times greater than that for type A, and the proportion of fuc in type A was 6 times higher than that in type B ⁄ C. The fuc derivative 4-keto-19¢-hexanoyloxyfucoxanthin (4-keto-hex) was present in type A but undetected in B ⁄ C. DNA sequencing of tufA distinguished morphotypes A, B ⁄ C (indistinguishable from B), and R, while little variation was observed within morphotypes. Thirty single nucleotide polymorphisms were identified in the 710 bp tufA sequence, of which 10 alleles were unique to B ⁄C and B morphotypes, seven alleles were unique to type A, and six alleles were unique to type R. We propose that the morphologically, physiologically, and genetically distinct Southern Ocean type B ⁄C sensu Young et al. (2003) be classified as E. huxleyi var. aurorae var. nov. S. S. Cook et Hallegr.

Item Details

Item Type:Refereed Article
Keywords:coccolithophorids, Southern Ocean
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Environment
Objective Group:Climate and Climate Change
Objective Field:Effects of Climate Change and Variability on Antarctic and Sub-Antarctic Environments (excl. Social Impacts)
Author:Cook, SS (Dr Suellen Cook)
Author:Whittock, LD (Dr Lucy Whittock)
Author:Wright, SW (Dr Simon Wright)
Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
ID Code:76294
Year Published:2011
Funding Support:Australian Research Council (DP1093801)
Web of Science® Times Cited:20
Deposited By:IMAS Research and Education Centre
Deposited On:2012-03-03
Last Modified:2012-05-24
Downloads:0

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