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A novel clade of Prochlorococcus found in high nutrient low chlorophyll waters in the South and Equatorial Pacific Ocean
Citation
West, NJ and Lebaron, P and Strutton, PG and Suzuki, MT, A novel clade of Prochlorococcus found in high nutrient low chlorophyll waters in the South and Equatorial Pacific Ocean, The ISME Journal: multidisciplinary journal of microbial ecology pp. 1-12. ISSN 1751-7362 (2011) [Refereed Article]
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Copyright Statement
© 2010 Nature Publishing Group
DOI: doi:10.1038/ismej.2010.186
Abstract
A novel high-light (HL)-adapted Prochlorococcus clade was discovered in high nutrient and low
chlorophyll (HNLC) waters in the South Pacific Ocean by phylogenetic analyses of 16S ribosomal
RNA (rRNA) and 16S23S internal transcribed spacer (ITS) sequences. This clade, named HNLC fell
within the HL-adapted Prochlorococcus clade with sequences above 99% similarity to one another,
and was divided into two subclades, HNLC1 and HNLC2. The distribution of the whole HNLC clade in
a northwest to southeast transect in the South Pacific (HNLC-to-gyre) and two 81N to 81S transects
in the Equatorial Pacific was determined by quantitative PCR using specific primers targeting ITS
regions. HNLC was the dominant HL Prochlorococcus clade (29% of bacterial 16S rRNA genes) at
the three westernmost stations in the South Pacific but decreased to less than 0.1% at the other
stations being replaced by the eMIT9312 ecotype in the hyperoligotrophic gyre. The highest
contributions of HNLC Prochlorococcus in both Equatorial Pacific transects along the latitudinal
lines of 1701W and 1551W were observed at the southernmost stations, reaching 16 and 6% of
bacterial 16S rRNA genes, respectively, whereas eMIT9312 dominated near the Equator. Spearman
Rank Order correlation analysis indicated that although both the HNLC clade and eMIT9312 were
correlated with temperature, they showed different correlations with regard to nutrients. HNLC only
showed significant correlations to ammonium uptake and regeneration rates, whereas eMIT9312
was negatively correlated with inorganic nutrients.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | biological oceanography |
| Research Division: | Earth Sciences |
| Research Group: | Oceanography |
| Research Field: | Biological Oceanography |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Earth Sciences |
| UTAS Author: | Strutton, PG (Associate Professor Peter Strutton) |
| ID Code: | 76100 |
| Year Published: | 2011 |
| Web of Science® Times Cited: | 29 |
| Deposited By: | IMAS Research and Education Centre |
| Deposited On: | 2012-02-28 |
| Last Modified: | 2012-04-03 |
| Downloads: | 0 |
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