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Characterization of mucus-associated proteins from abalone (Haliotis) - candidates for chemical signaling

Citation

Kuanpradit, C and Stewart, MJ and York, PS and Degnan, BM and Sobhon, P and Hanna, PJ and Chavadej, J and Cummins, SF, Characterization of mucus-associated proteins from abalone (Haliotis) - candidates for chemical signaling, The FEBS Journal, 279, (3) pp. 437-450. ISSN 1742-464X (2012) [Refereed Article]


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The definitive published version is available online at: http://www3.interscience.wiley.com/

DOI: doi:10.1111/j.1742-4658.2011.08436.x

Abstract

Living in groups is a widespread phenomenon in the animal kingdom. For free-spawning aquatic animals, such as the abalone (Haliotis), being in the close proximity to potential mating partners enhances reproductive success. In this study, we investigated whether chemical cues could be present in abalone mucus that enable species-specific aggregation. A comparative MS analysis of mucus obtained from trailing or fixed stationary Haliotis asinina, and from seawater surrounding aggregations, indicated that watersoluble biomolecules are present and that these can stimulate sensory activity in conspecifics. Purified extracts of trail mucus contain at least three small proteins [termed H. asinina mucus-associated proteins (Has-MAPs)- 1–3], which readily diffuse into the surrounding seawater and evoke a robust cephalic tentacle response in conspecifics. Mature Has-MAP-1 is approximately 9.9 kDa in size, and has a glycine-rich N-terminal region. Has-MAP-2 is approximately 6.2 kDa in size, and has similarities to schistosomin, a protein that is known to play a role in mollusc reproduction. The mature Has-MAP-3 is approximately 12.5 kDa in size, and could only be identified within trail mucus of animals outside of the reproductive season. All three Has-MAP genes are expressed at high levels within secretory cells of the juvenile abalone posterior pedal gland, consistent with a role in scent marking. We infer from these results that abalone mucus-associated proteins are candidate chemical cues that could provide informational cues to conspecifics living in close proximity and, given their apparent stability and hydrophilicity, animals further afield.

Item Details

Item Type:Refereed Article
Keywords:abalone, animal aggregation, Haliotis, mucus-associated proteins, RP-HPLC
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Invertebrate Biology
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Molluscs (excl. Oysters)
Author:Stewart, MJ (Dr Michael Stewart)
ID Code:76050
Year Published:2012
Web of Science® Times Cited:12
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2012-02-24
Last Modified:2013-05-06
Downloads:0

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