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Studies on triploid oysters in Australia: evaluation of cytochalasin B and 6-dimethylaminopurine for triploidy induction in Sydney rock oysters Saccostrea commercialis (Iredale and Roughley)

Citation

Nell, JA and Hand, RE and Goard, LJ and McAdam, SP and Maguire, GB, Studies on triploid oysters in Australia: evaluation of cytochalasin B and 6-dimethylaminopurine for triploidy induction in Sydney rock oysters Saccostrea commercialis (Iredale and Roughley), Aquaculture Research, 27, (9) pp. 689-698. ISSN 1355-557X (1996) [Refereed Article]

DOI: doi:10.1046/j.1365-2109.1996.t01-1-00777.x

Abstract

Naturally spawned Sydney rock oysters Saccostrea commercialis (Iredale and Roughley), were used to determine the appropriate stage of development for inducing triploidy and to compare the effectiveness of cytochalasin B (CB) and 6-dimethylaminopurine (6-DMAP) in dose-optimization trials. Induction should commence at 50% first polar body (PB1) extrusion in eggs (approximately 17-19 min post-fertilization at 25°C). By day 5 the highest triploidy percentage and yield (number of triploid larvae per 100 fertilized eggs) were achieved in the ranges of 0.75-1.5 mg CB l-1 (1.6- 3.1 μM CB) or 200-400 μM 6-DMAP (32.6-65.3 mg 6-DMAP l-1). However, CB treatment resulted in greater survival and triploidy percentage than 6-DMAP in Sydney rock oysters.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Other Animal Production and Animal Primary Products
Objective Field:Animal Production and Animal Primary Products not elsewhere classified
Author:Nell, JA ( Nell)
Author:Maguire, GB (Dr Greg Maguire)
ID Code:7591
Year Published:1996
Deposited By:TAFI - Aquaculture
Deposited On:1996-08-01
Last Modified:2011-09-20
Downloads:0

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