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Isolation and characterization of 52 polymorphic EST-SSR markers for Callitris columellaris (Cupressaceae)

Citation

Sakaguchi, S and Uchiyama, K and Ueno, S and Ujino-Ihara, T and Tsumura, Y and Prior, LD and Bowman, DMJS and Crisp, MD and Isagi, Y, Isolation and characterization of 52 polymorphic EST-SSR markers for Callitris columellaris (Cupressaceae), American Journal of Botany, 98, (12) pp. e363-e368. ISSN 0002-9122 (2011) [Refereed Article]


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Copyright Statement

Copyright 2011 CSIRO

DOI: doi:10.3732/ajb.1100276

Abstract

Premise of the study: We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Callitris columellaris sensu lato (s.l.) to elucidate population genetic structure and detect outlier loci by genome scan. Methods and Results: mRNA from an individual seedling was subjected to cDNA synthesis and then de novo pyrosequencing. Two hundred and nineteen primer pairs bordering sequence regions were designed from the obtained sequence data. In total, 52 showed polymorphism within 16 individuals representative of the species entire range, with the number of alleles per locus and expected heterozygosity ranging from two to 10 and 0.06 to 0.84, respectively. Conclusions: The EST-SSR markers developed in this study will be useful for evaluating the range-wide genetic structure of C. columellaris s.l. and detecting outlier loci under selection, as well as providing useful markers to investigate the conservation genetics and reproductive ecology of the species.

Item Details

Item Type:Refereed Article
Keywords:Callitris columellaris ; Cupressaceae; expressed sequence tag; genetic structure; microsatellite; pyrosequencing.
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Population, Ecological and Evolutionary Genetics
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Forest and Woodlands Flora, Fauna and Biodiversity
Author:Prior, LD (Dr Lynda Prior)
Author:Bowman, DMJS (Professor David Bowman)
ID Code:75885
Year Published:2011
Web of Science® Times Cited:6
Deposited By:Plant Science
Deposited On:2012-02-20
Last Modified:2017-11-01
Downloads:0

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