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Isolation and characterization of 52 polymorphic EST-SSR markers for Callitris columellaris (Cupressaceae)
Citation
Sakaguchi, S and Uchiyama, K and Ueno, S and Ujino-Ihara, T and Tsumura, Y and Prior, LD and Bowman, DMJS and Crisp, MD and Isagi, Y, Isolation and characterization of 52 polymorphic EST-SSR markers for Callitris columellaris (Cupressaceae), American Journal of Botany, 98, (12) pp. e363-e368. ISSN 0002-9122 (2011) [Refereed Article]
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Copyright Statement
Copyright © 2011 CSIRO
Abstract
• Premise of the study: We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Callitris
columellaris sensu lato (s.l.) to elucidate population genetic structure and detect outlier loci by genome scan.
• Methods and Results: mRNA from an individual seedling was subjected to cDNA synthesis and then de novo pyrosequencing.
Two hundred and nineteen primer pairs bordering sequence regions were designed from the obtained sequence data. In total,
52 showed polymorphism within 16 individuals representative of the species ’ entire range, with the number of alleles per locus
and expected heterozygosity ranging from two to 10 and 0.06 to 0.84, respectively.
• Conclusions: The EST-SSR markers developed in this study will be useful for evaluating the range-wide genetic structure of
C. columellaris s.l. and detecting outlier loci under selection, as well as providing useful markers to investigate the conservation
genetics and reproductive ecology of the species.
Item Details
Item Type: | Refereed Article |
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Keywords: | Callitris columellaris ; Cupressaceae; expressed sequence tag; genetic structure; microsatellite; pyrosequencing. |
Research Division: | Biological Sciences |
Research Group: | Genetics |
Research Field: | Genetics not elsewhere classified |
Objective Division: | Environmental Management |
Objective Group: | Terrestrial systems and management |
Objective Field: | Terrestrial biodiversity |
UTAS Author: | Prior, LD (Dr Lynda Prior) |
UTAS Author: | Bowman, DMJS (Professor David Bowman) |
ID Code: | 75885 |
Year Published: | 2011 |
Web of Science® Times Cited: | 8 |
Deposited By: | Plant Science |
Deposited On: | 2012-02-20 |
Last Modified: | 2017-11-01 |
Downloads: | 0 |
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