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Testing Hygrometers Used in Cytogenetics Laboratories for Metaphase Preparation
Citation
Hartley, TF and Dun, K, Testing Hygrometers Used in Cytogenetics Laboratories for Metaphase Preparation, Current Protocols in Human Genetics, (Jul) pp. A.2F.1-A.2F.8. ISSN 1934-8258 (2011) [Refereed Article]
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DOI: doi:10.1002/0471142905.hga02fs70
Abstract
This protocol describes procedures for checking small laboratory hygrometers for accuracy at three relative humidity (rh) levels. The work arose out of the need to provide laboratory assessors with documentary evidence that the hygrometer used to monitor humidity in the vicinity of the laboratory where medical cytogenetics testing slides are prepared and dried in the ambient environment is reproducible and sufficiently accurate. The procedure is based upon the physicochemical principle that when water or certain saturated salt solutions are placed into a sealed environment, the humidity will equilibrate to well defined levels. We choose to check our hygrometers at three points: 95%, 75%, and 33% rh, using distilled water, saturated sodium chloride solution, and saturated magnesium chloride solution, respectively. Our results have demonstrated that the procedure is convenient and of sufficient accuracy to be fit for this annual hygrometer validation purpose. The procedure takes 24 hr per relative humidity point checked.
Item Details
Item Type: | Refereed Article |
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Keywords: | Hygrometer check - ISO 15189 - slide drying - environmental humidity - chromosome spreading - chromosome crossovers |
Research Division: | Biomedical and Clinical Sciences |
Research Group: | Clinical sciences |
Research Field: | Pathology (excl. oral pathology) |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Diagnosis of human diseases and conditions |
UTAS Author: | Hartley, TF (Dr Thomas Hartley) |
ID Code: | 75712 |
Year Published: | 2011 |
Deposited By: | Health Sciences A |
Deposited On: | 2012-02-10 |
Last Modified: | 2012-06-21 |
Downloads: | 0 |
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