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Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting


Ryan, KG and Tay, ML and Martin, AR and McMinn, A and Davy, SK, Chlorophyll fluorescence imaging analysis of the responses of Antarctic bottom-ice algae to light and salinity during melting, Journal of Experimental Marine Biology and Ecology, 399, (2) pp. 156-161. ISSN 0022-0981 (2011) [Refereed Article]

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DOI: doi:10.1016/j.jembe.2011.01.006


Bottom-ice algae within Antarctic sea ice were examined using chlorophyll fluorescence imaging. The detailed structure of the bottom-ice algal community growing in the platelet and congelation layers of solid pieces of sea ice was evident for the first time in chlorophyll imaging mode. Strands of fluorescence representing algal cells were clearly visible growing upward into brine channels in a fine network. Images of effective quantum yield (ÔPSII) revealed that theÔPSII of algae embedded in the sea ice was approximately 0.5. Furthermore,ÔPSII decreased slightly with distance from the ice–water interface. The response of Antarctic sea ice algae to changes in irradiance and salinity, and the effects of slowly warming and melting the ice block sample were examined using this system. The ÔPSII of bottom-ice algae decreased as irradiance increased and salinities decreased. Bottom-ice algae appear to be most vulnerable to changes in their environment during the melting process of the ice, and this suggests that algae from this region of the ice may not be able to cope with the stress of melting during summer. Chlorophyll fluorescence imaging provides unprecedented imagery of chlorophyll distribution in sea ice and allows measurement of the responses of sea ice algae to environmental stresses with minimal disruption to their physical habitat. The results obtained with this method are comparable to those obtained with algae that have been melted into liquid culture and this indicates that previous melting protocols reveal meaningful data. In this chlorophyll imaging study, rapid light curves did not saturate and this may prevent further use of this configuration.

Item Details

Item Type:Refereed Article
Keywords:Antarctic sea ice algae salinity UV
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Marine and estuarine ecology (incl. marine ichthyology)
Objective Division:Environmental Management
Objective Group:Management of Antarctic and Southern Ocean environments
Objective Field:Biodiversity in Antarctic and Southern Ocean environments
UTAS Author:Martin, AR (Dr Andrew Martin)
UTAS Author:McMinn, A (Professor Andrew McMinn)
ID Code:75594
Year Published:2011
Funding Support:Australian Research Council (DP0773558)
Web of Science® Times Cited:23
Deposited By:IMAS Research and Education Centre
Deposited On:2012-02-06
Last Modified:2012-04-12

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