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Gelatinase A possesses a beta-secretase-like activity in cleaving the amyloid protein precursor of Alzheimer's disease

Citation

LePage, RN and Fosang, AJ and Fuller, SJ and Murphy, G and Evin, G and Beyreuther, K and Masters, CL and Small, DH, Gelatinase A possesses a beta-secretase-like activity in cleaving the amyloid protein precursor of Alzheimer's disease, F E B S Letters: (Federation of European Biochemical Societies), 377, (2) pp. 267-270. ISSN 0014-5793 (1995) [Refereed Article]


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DOI: doi:10.1016/0014-5793(95)01358-X

Abstract

The ability of the 72 kDa gelatinase A to cleave the amyloid protein precursor (APP) was investigated. HeLa cells were transfected with an APP695 plasmid. The cells were incubated with gelatinase A, which cleaved the 110 kDa cell-surface APP, releasing a 100 kDa form of the protein. A peptide homologous to the β-secretase site was cleaved by gelatinase A adjacent to a glutamate residue at position −3 (βA4 numbering system). A peptide homologous to the α-secretase site was not cleaved. The results demonstrate that 72 kDa gelatinase A is not an α-secretase, but that it may have a β-secretase activity.

Item Details

Item Type:Refereed Article
Keywords: Alzheimer's disease; Amyloid; Gelatinase; Secretase; Protease
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Neurodegenerative Disorders Related to Ageing
Author:Small, DH (Professor David Small)
ID Code:75421
Year Published:1995
Web of Science® Times Cited:35
Deposited By:Research Division
Deposited On:2012-01-30
Last Modified:2012-02-01
Downloads:0

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