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Recombinant human amyloid precursor-like protein 2 (APLP2) expressed in the yeast Pichia pastoris can stimulate neurite outgrowth

Citation

Cappai, R and Mok, SS and Galatis, D and Tucker, DF and Henry, A and Beyreuther, K and Small, DH and Masters, CL, Recombinant human amyloid precursor-like protein 2 (APLP2) expressed in the yeast Pichia pastoris can stimulate neurite outgrowth, F E B S Letters: (Federation of European Biochemical Societies), 442, (1) pp. 95-98. ISSN 0014-5793 (1999) [Refereed Article]


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DOI: doi:10.1016/S0014-5793(98)01635-4

Abstract

The human amyloid precursor-like protein 2 (APLP2) is a member of the Alzheimer's disease amyloid precursor protein (APP) gene family. The human APLP2 ectodomain (sAPLP2) was expressed in the yeast Pichia pastoris and the recombinant sAPLP2 was purified from the culture medium in a single step by metal-chelating Sepharose chromatography. The neuritotrophic activity of APLP2 was compared to the APP isoforms sAPP695 and sAPP751 on chick sympathetic neurones. APLP2 had neurite outgrowth-promoting activity similar to that of the APP isoforms. This suggests that APP and APLP2 have a similar or related role and supports the idea of a redundancy in function between the APP-gene family proteins.

Item Details

Item Type:Refereed Article
Keywords: Amyloid precursor-like protein 2; Neuritogenesis
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Neurodegenerative Disorders Related to Ageing
Author:Small, DH (Professor David Small)
ID Code:75403
Year Published:1999
Web of Science® Times Cited:43
Deposited By:Research Division
Deposited On:2012-01-30
Last Modified:2012-02-02
Downloads:0

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