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Wheat germ agglutinin-binding glycoproteins are decreased in Alzheimer's disease cerebrospinal fluid
journal contribution
posted on 2023-05-17, 10:00 authored by Fodero, LR, Saez-Valero, J, Barquero, MS, Marcos, A, McLean, CA, David SmallDavid SmallA number of biomarkers (e.g. Aβ, tau) has been identified in Alzheimer's disease CSF. However, none fulfils the criteria of sensitivity and specificity (> 80%) needed for the development of an accurate diagnostic test. The lack of a suitable marker has prompted the search for new CSF biomarkers. In this study, the glycosylation of CSF proteins was examined using lectin blotting. Lumbar CSF was collected ante mortem from 22 non-Alzheimer's disease and 12 probable Alzheimer's disease cases and ventricular CSF collected post mortem from 7 non-Alzheimer's disease and 16 Alzheimer's disease cases confirmed by pathologic examination. When CSF glycoproteins were stained with wheat germ agglutinin (WGA), the staining intensity was found to be significantly lower in the Alzheimer's disease group. No difference in staining was found using other lectins (Canavalia ensiformis agglutinin, Ricinus communis agglutinin, Lens culinaris agglutinin). The measurement of WGA-reactive glycoproteins in CSF may be a useful biomarker for diagnosis of Alzheimer's disease.
History
Publication title
Journal of NeurochemistryVolume
79Issue
5Pagination
1022-1026ISSN
0022-3042Department/School
Menzies Institute for Medical ResearchPublisher
Wiley-BlackwellPlace of publication
United KingdomRights statement
The definitive published version is available online at: http://www3.interscience.wiley.com/Repository Status
- Restricted