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Wheat germ agglutinin-binding glycoproteins are decreased in Alzheimer's disease cerebrospinal fluid


Fodero, LR and Saez-Valero, J and Barquero, MS and Marcos, A and McLean, CA and Small, DH, Wheat germ agglutinin-binding glycoproteins are decreased in Alzheimer's disease cerebrospinal fluid, Journal of Neurochemistry, 79, (5) pp. 1022-1026. ISSN 0022-3042 (2001) [Refereed Article]

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DOI: doi:10.1046/j.1471-4159.2001.00640.x


A number of biomarkers (e.g. Aβ, tau) has been identified in Alzheimer's disease CSF. However, none fulfils the criteria of sensitivity and specificity (> 80%) needed for the development of an accurate diagnostic test. The lack of a suitable marker has prompted the search for new CSF biomarkers. In this study, the glycosylation of CSF proteins was examined using lectin blotting. Lumbar CSF was collected ante mortem from 22 non-Alzheimer's disease and 12 probable Alzheimer's disease cases and ventricular CSF collected post mortem from 7 non-Alzheimer's disease and 16 Alzheimer's disease cases confirmed by pathologic examination. When CSF glycoproteins were stained with wheat germ agglutinin (WGA), the staining intensity was found to be significantly lower in the Alzheimer's disease group. No difference in staining was found using other lectins (Canavalia ensiformis agglutinin, Ricinus communis agglutinin, Lens culinaris agglutinin). The measurement of WGA-reactive glycoproteins in CSF may be a useful biomarker for diagnosis of Alzheimer's disease.

Item Details

Item Type:Refereed Article
Keywords: Alzheimer's disease; CSF; glycoproteins; WGA
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Small, DH (Professor David Small)
ID Code:75359
Year Published:2001
Web of Science® Times Cited:31
Deposited By:Research Division
Deposited On:2012-01-25
Last Modified:2012-02-02

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