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Ontogenic and sexually dimorphic expression of cyp19 isoforms in the rainbowfish, Melanotaenia fluviatilis (Castelnau 1878)
Citation
Shanthanagouda, AH and Patil, JG and Nugegoda, D, Ontogenic and sexually dimorphic expression of cyp19 isoforms in the rainbowfish, Melanotaenia fluviatilis (Castelnau 1878), Comparative Biochemistry and Physiology. Part A: Molecular and Integrative Physiology, 161, (2) pp. 250-258. ISSN 1095-6433 (2012) [Refereed Article]
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DOI: doi:10.1016/j.cbpa.2011.11.006
Abstract
To investigate the role of cytochrome P450 aromatase, we isolated cyp19 isoforms in the Murray
River rainbowfish, M. fluviatilis. The cloned cDNA for cyp19a1a and cyp19a1b had an open
reading frame (ORF) of 492 and 499 amino acid residues, with shared identity of up to 83% and
87% with the corresponding homologues of other teleosts respectively. In contrast, the cyp19a1a
and cyp19a1b of the Murray River rainbowfish had a shared identity of only 61%. Not surprisingly,
the phylogenetic analysis clustered the M. fluviatilis cyp19 isoforms with the corresponding
isoforms of other teleosts, suggesting a shared evolutionary ancestry of the respective isoforms. We
also studied the expression of cyp19 isoforms during ontogeny and in adult fish using quantitative
Real-Time PCR (qPCR). Results suggest that uniquely only cyp19a1b transcripts are maternally
inherited, suggesting its role in early development and growth in the species. In contrast to reports
in many teleosts, the cyp19a1a was exclusively expressed in the ovarian tissue and completely
absent in other tissues examined, including testis. The cyp19a1b like in most teleosts was predominantly expressed
in the brain of both males and females with low level of expression in other tissues including gonads of
both sexes.
Item Details
Item Type: | Refereed Article |
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Keywords: | aromatase, maternal inheritance, Australian native species |
Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Fisheries sciences |
Research Field: | Fish physiology and genetics |
Objective Division: | Environmental Management |
Objective Group: | Marine systems and management |
Objective Field: | Assessment and management of benthic marine ecosystems |
UTAS Author: | Patil, JG (Dr Jawahar Patil) |
ID Code: | 74549 |
Year Published: | 2012 |
Web of Science® Times Cited: | 17 |
Deposited By: | NC Marine Conservation and Resource Sustainability |
Deposited On: | 2011-12-07 |
Last Modified: | 2017-10-31 |
Downloads: | 0 |
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