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Tumor-specific diagnostic marker for transmissible facial tumors of Tasmanian devils: immunohistochemistry studies

Citation

Tovar, C and Obendorf, D and Murchison, EP and Papenfuss, AT and Kreiss, A and Woods, GM, Tumor-specific diagnostic marker for transmissible facial tumors of Tasmanian devils: immunohistochemistry studies, Veterinary Pathology, 48, (6) pp. 1195-1203. ISSN 1544-2217 (2011) [Refereed Article]


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Copyright Statement

Copyright 2011 The American College of Veterinary Pathologists

DOI: doi:10.1177/0300985811400447

Abstract

Devil facial tumor disease (DFTD) is a transmissible neoplasm that is threatening the survival of the Tasmanian devil. Genetic analyses have indicated that the disease is a peripheral nerve sheath neoplasm of Schwann cell origin. DFTD cells express genes characteristic of myelinating Schwann cells, and periaxin, a Schwann cell protein, has been proposed as a marker for the disease. Diagnosis of DFTD is currently based on histopathology, cytogenetics, and clinical appearance of the disease in affected animals. As devils are susceptible to a variety of neoplastic processes, a specific diagnostic test is required to differentiate DFTD from cancers of similar morphological appearance. This study presents a thorough examination of the expression of a set of Schwann cell and other neural crest markers in DFTD tumors and normal devil tissues. Samples from 20 primary DFTD tumors and 10 DFTD metastases were evaluated by immunohistochemistry for the expression of periaxin, S100 protein, peripheral myelin protein 22, nerve growth factor receptor, nestin, neuron specific enolase, chromogranin A, and myelin basic protein. Of these, periaxin was confirmed as the most sensitive and specific marker, labeling the majority of DFTD cells in 100% of primary DFTD tumors and DFTD metastases. In normal tissues, periaxin showed specificity for Schwann cells in peripheral nerve bundles. This marker was then evaluated in cultured devil Schwann cells, DFTD cell lines, and xenografted DFTD tumors. Periaxin expression was maintained in all these models, validating its utility as a diagnostic marker for the disease.

Item Details

Item Type:Refereed Article
Keywords:devil facial tumor disease, DFTD, immunohistochemistry, periaxin, Tasmanian devil
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Humoural Immunology and Immunochemistry
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
Author:Tovar, C (Dr Cesar Tovar Lopez)
Author:Obendorf, D (Dr David Obendorf)
Author:Kreiss, A (Dr Alexandre Kreiss)
Author:Woods, GM (Professor Gregory Woods)
ID Code:74331
Year Published:2011
Web of Science® Times Cited:24
Deposited By:Menzies Institute for Medical Research
Deposited On:2011-11-22
Last Modified:2015-09-01
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