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The development of a fluorescent antibody stain to identify a Uronema sp. (Ciliophora:Scuticociliatida) implicated in fatal encephalitis in southern bluefin tuna (Thunnus Maccoyii)

Citation

Watts, M and Burke, CM and Munday, BL, The development of a fluorescent antibody stain to identify a Uronema sp. (Ciliophora:Scuticociliatida) implicated in fatal encephalitis in southern bluefin tuna (Thunnus Maccoyii), Bulletin of the European Association of Fish Pathologists, 16, (3) pp. 104-108. ISSN 0108-0288 (1996) [Refereed Article]

Abstract

Polyclonal antisera, designated anti-Q antiserum and anti-B antiserum, were produced in rabbits against Uronema sp. isolated from diseased tuna (Q) and dead blenny larvae (B). Extraneous bacterial antibodies and cross-reacting ciliate antibodies were present in the antiserum and were removed by adsorption, enabling Uronema sp. to be easily differentiated within a marine ciliate assemblage. Adsorbed anti-B antiserum, diluted to 1/50, was used in indirect fluorescent antibody tests (IFATs) and detected ≥ 10 Uronema sp. in a 1 L sample of seawater and approximately 10 presumptive Uronema/15 μL in sediment samples from a tuna sea-cage. In addition, a variable staining pattern was demonstrated with different isolates of Uronema sp. The results indicate that this test is able to detect both cultured and environmental Uronema sp. and may have an application both as an environmental monitoring tool and a means of speciating Uronema.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Environment
Objective Group:Other Environment
Objective Field:Environment not elsewhere classified
Author:Watts, M (Dr Marianne Watts)
Author:Burke, CM (Dr Chris Burke)
Author:Munday, BL (Dr Barry Munday)
ID Code:7426
Year Published:1996
Deposited By:Health Sciences A
Deposited On:1996-08-01
Last Modified:2011-08-19
Downloads:0

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