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Novel application of a fish gill cell line assay to assess ichthyotoxicity of harmful marine microalgae
Citation
Dorantes Aranda, JJ and Waite, TD and Godrant, A and Rose, AL and Tovar, CD and Woods, GM and Hallegraeff, GM, Novel application of a fish gill cell line assay to assess ichthyotoxicity of harmful marine microalgae, Harmful Algae, 10, (4) pp. 366-373. ISSN 1568-9883 (2011) [Refereed Article]
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DOI: doi:10.1016/j.hal.2011.01.002
Abstract
Fish-killingmicroalgae can cause substantial mortalities of cultured finfish, but their killing mechanisms
are not completely understood. Since use of cell lines offers significant advantages compared to working
with whole organisms, a simple in vitro assay for microalgal ichthyotoxicity is described using the
rainbow trout cell line RTgill-W1. We describe the application of a microplate based assay for testing the
toxicity of fatty acids to gill cells in a time-dependent manner. Additionally, a modification of this assay
using an insert support with permeable membranes is presented to test the toxicity of living marine
microalgae. The endpoint ismeasurement of cell viability using alamarBlue as an indicator dye. Gill cells
remained 100% viable for 60 h in the modified version of the basal growth medium, L-15/ex, without
fetal bovine serum and with 1% methanol (in which fatty acids were dissolved). In contrast, gill cells
survived for 3 h in microplates with insert supports upon exposure to algal seawater-based media.
Toxicity of palmitic acid was less than 10%, as observed by loss of gill cell viability, during the first 12 h at
all concentrations. However, the highest toxicity was observed at 48–60 h with a reduction of cell
viability of 50% after exposure to 140 mg L1. The ichthyotoxic raphidophyte Chattonella marina and
dinoflagellate Karlodinium veneficum had a negative effect on gill cells, causing up to 70 and 38% loss of
viability, respectively, when exposed to high algal concentrations for 2 h. These two simple and
reproducible screening tests represent a sensitive and suitable alternative to whole fish ichthyotoxic
bioassays for fish-killing marine microalgae.
Item Details
Item Type: | Refereed Article |
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Keywords: | ichthyotoxicity, harmful phytoplankton, Chattonella marina, Karlodinium veneficum, rainbow trout gill cell line, fatty acids |
Research Division: | Agricultural and Veterinary Sciences |
Research Group: | Fisheries Sciences |
Research Field: | Aquaculture |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - Aquaculture |
Objective Field: | Aquaculture Fin Fish (excl. Tuna) |
UTAS Author: | Dorantes Aranda, JJ (Dr Juan Dorantes Aranda) |
UTAS Author: | Tovar, CD (Dr Cesar Tovar Lopez) |
UTAS Author: | Woods, GM (Professor Gregory Woods) |
UTAS Author: | Hallegraeff, GM (Professor Gustaaf Hallegraeff) |
ID Code: | 74233 |
Year Published: | 2011 |
Web of Science® Times Cited: | 32 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2011-11-15 |
Last Modified: | 2017-11-03 |
Downloads: | 2 View Download Statistics |
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